| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,481,284 | T→G | 34.2% | A362A (GCT→GCG) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,481,284 | 0 | T | G | 34.2% | 63.2 / 61.5 | 79 | A362A (GCT→GCG) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (17/35); new base G (20/7); total (37/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.48e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-03 | |||||||||||
CGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTG > minE/1481219‑1481342 | ggcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:1085324/66‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:1045210/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:2368819/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:2203096/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:21939/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:2593614/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:2055344/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:2035998/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:2007900/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:1989093/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:3120416/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:3167952/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:1703422/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:3302229/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:160457/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:775701/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:717609/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:624980/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:122497/67‑1 (MQ=255)cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTg < 1:556618/67‑1 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:3425078/1‑68 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:674748/1‑68 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:2160957/1‑68 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:2971005/1‑68 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:362531/1‑68 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:1938256/1‑68 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:353402/1‑68 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:3157910/1‑68 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATgg > 1:1410876/1‑68 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:3031156/65‑1 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:3116875/65‑1 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:1006317/65‑1 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:2453045/65‑1 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:251448/65‑1 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:1147229/65‑1 (MQ=255) atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:1901328/65‑1 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGc > 1:860095/1‑52 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGAcgccg > 1:2239019/1‑61 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGAcgccg > 1:1172525/1‑61 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGAcgccg > 1:3147149/1‑61 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:2342331/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:981867/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:871866/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:781810/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:1058631/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:501529/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:1616339/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:3271032/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:1867345/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:3046202/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:2345436/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:271791/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:2850545/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGTATga > 1:2243668/1‑67 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGAGGAATGGGCTACCGACGCCGt > 1:1512343/1‑64 (MQ=255) tcacaatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:1265165/1‑64 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGCCGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:1294954/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2842978/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2376815/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:821093/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2476118/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2168118/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:1747186/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:635198/1‑69 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc < 1:658572/68‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc < 1:821843/68‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:3136174/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:2643409/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:2787748/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:808827/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:1795713/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:3408221/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:900938/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:296483/69‑1 (MQ=255) aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:184107/68‑1 (MQ=255) aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:2808916/68‑1 (MQ=255) ggCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTg < 1:2018967/69‑1 (MQ=255) ggCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTg < 1:1139014/69‑1 (MQ=255) ggCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTg < 1:1325981/69‑1 (MQ=255) | CGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTG > minE/1481219‑1481342 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |