| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,481,302 | G→C | 46.9% | A368A (GCG→GCC) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,481,302 | 0 | G | C | 46.9% | 1.2 / 55.6 | 49 | A368A (GCG→GCC) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (11/15); new base C (16/7); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.48e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
ATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCC > minE/1481240‑1481358 | atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:1006317/65‑1 (MQ=255)atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:1147229/65‑1 (MQ=255)atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:2453045/65‑1 (MQ=255)atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:1901328/65‑1 (MQ=255)atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:251448/65‑1 (MQ=255)atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:3116875/65‑1 (MQ=255)atcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt < 1:3031156/65‑1 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:2342331/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:1058631/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:2345436/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:271791/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:2850545/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:3046202/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:3271032/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:501529/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:781810/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:871866/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:981867/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:1867345/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:1616339/1‑64 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGTATga > 1:2243668/1‑67 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGAGGAATGGGCTACCGACGCCGt > 1:1512343/1‑64 (MQ=255) tcacaatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCGGAATGGGCTACCGACGCCGt > 1:1265165/1‑64 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGCCGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:1294954/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2168118/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:821093/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:635198/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:1747186/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2842978/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2376815/1‑69 (MQ=255) cTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2476118/1‑69 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc < 1:821843/68‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc < 1:658572/68‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:1795713/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:296483/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:2787748/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:3136174/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:900938/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:3408221/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:2643409/69‑1 (MQ=255) tATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:808827/69‑1 (MQ=255) aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:2808916/68‑1 (MQ=255) aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:184107/68‑1 (MQ=255) ggCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTg < 1:1139014/69‑1 (MQ=255) ggCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTg < 1:2018967/69‑1 (MQ=255) ggCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTg < 1:1325981/69‑1 (MQ=255) ggCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGcc > 1:593250/1‑69 (MQ=255) ggCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGcc > 1:1318359/1‑69 (MQ=255) ggCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGcc > 1:3386506/1‑69 (MQ=255) | ATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCC > minE/1481240‑1481358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |