| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,486,331 | C→T | 31.6% | P327P (CCG→CCA) | nuoN ← | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,486,331 | 0 | C | T | 31.6% | 69.7 / 38.0 | 80 | P327P (CCG→CCA) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base C (15/39); new base T (7/18); total (22/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
AGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAG > minE/1486263‑1486394 | aGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATc > 1:815670/1‑69 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:2981004/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:1785829/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:1789374/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:1765770/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:3425503/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:382401/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:1736760/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:390162/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:1863837/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:2015344/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:265347/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:2229970/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:829550/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:128819/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:983495/69‑1 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:2337284/69‑1 (MQ=255) caAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATa > 1:2341722/1‑67 (MQ=255) caAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATAcc > 1:1775464/1‑69 (MQ=255) caAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATAcc > 1:516716/1‑69 (MQ=255) caAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATAcc > 1:721254/1‑69 (MQ=255) gACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGCCATCgg < 1:3464/51‑1 (MQ=255) cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc < 1:351012/68‑1 (MQ=255) cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc < 1:315940/68‑1 (MQ=255) cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc < 1:2984052/68‑1 (MQ=255) cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc < 1:2940157/68‑1 (MQ=255) cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc < 1:1509149/68‑1 (MQ=255) cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc < 1:2638094/68‑1 (MQ=255) cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc < 1:939873/68‑1 (MQ=255) accgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc < 1:1019412/67‑1 (MQ=255) cgaccgacAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc > 1:1811262/1‑68 (MQ=255) cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc > 1:1042090/1‑68 (MQ=255) cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc > 1:3337339/1‑68 (MQ=255) cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc > 1:554576/1‑68 (MQ=255) cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc > 1:709385/1‑68 (MQ=255) cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc > 1:2259088/1‑68 (MQ=255) cgaccgCCAGCAAGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc > 1:1855480/1‑68 (MQ=255) gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat > 1:2869363/1‑69 (MQ=255) gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat > 1:359436/1‑69 (MQ=255) gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat > 1:3415101/1‑69 (MQ=255) gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat > 1:1871471/1‑69 (MQ=255) gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat > 1:743941/1‑69 (MQ=255) gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat > 1:2772058/1‑69 (MQ=255) gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat > 1:2771509/1‑69 (MQ=255) gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat > 1:1234120/1‑69 (MQ=255) accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc < 1:2149008/69‑1 (MQ=255) accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc < 1:1752639/69‑1 (MQ=255) accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc < 1:1837477/69‑1 (MQ=255) accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc < 1:911567/69‑1 (MQ=255) accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc < 1:142734/69‑1 (MQ=255) accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc < 1:3211541/69‑1 (MQ=255) accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc < 1:2914722/69‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCGAGCGTCATTGGGATACCAGCCAgtga < 1:679566/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:1631934/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:1665669/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:453661/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:1748749/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:897121/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:1043650/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:1439900/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:801216/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:777245/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:348246/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:2006058/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:2051537/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:2128440/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:2562807/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:2591723/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:277232/57‑1 (MQ=255) cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga < 1:2700952/57‑1 (MQ=255) cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGtcat < 1:1688249/69‑1 (MQ=255) cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGtcat < 1:225859/69‑1 (MQ=255) cGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCCTCATCACCGTCATCACTGCCGCGAgg < 1:3239007/68‑1 (MQ=255) cGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAgg < 1:2614607/68‑1 (MQ=255) gATAAAGCCCAGCGTCATGGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAgg < 1:2379509/67‑1 (MQ=255) gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGAc < 1:2376528/68‑1 (MQ=255) gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGAc < 1:1572077/68‑1 (MQ=255) gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGAc < 1:594996/68‑1 (MQ=255) tCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAg > 1:1447005/1‑68 (MQ=255) tCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAg > 1:2402149/1‑68 (MQ=255) tCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAg > 1:1920881/1‑68 (MQ=255) | AGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAG > minE/1486263‑1486394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |