Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,486,340 G→A 40.9% A324A (GCC→GCT nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,486,3400GA40.9% 23.7 / 40.5 61A324A (GCC→GCTnuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base G (13/23);  new base A (7/18);  total (20/41)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGC  >  minE/1486285‑1486404
                                                       |                                                                
cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc                                                      <  1:939873/68‑1 (MQ=255)
cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc                                                      <  1:351012/68‑1 (MQ=255)
cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc                                                      <  1:1509149/68‑1 (MQ=255)
cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc                                                      <  1:315940/68‑1 (MQ=255)
cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc                                                      <  1:2984052/68‑1 (MQ=255)
cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc                                                      <  1:2940157/68‑1 (MQ=255)
cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc                                                      <  1:2638094/68‑1 (MQ=255)
 accgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGc                                                      <  1:1019412/67‑1 (MQ=255)
   cgaccgacAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc                                                   >  1:1811262/1‑68 (MQ=255)
   cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc                                                   >  1:1042090/1‑68 (MQ=255)
   cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc                                                   >  1:709385/1‑68 (MQ=255)
   cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc                                                   >  1:554576/1‑68 (MQ=255)
   cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc                                                   >  1:3337339/1‑68 (MQ=255)
   cgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc                                                   >  1:2259088/1‑68 (MQ=255)
   cgaccgCCAGCAAGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAGTGAAAGcatc                                                   >  1:1855480/1‑68 (MQ=255)
    gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat                                                 >  1:1871471/1‑69 (MQ=255)
    gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat                                                 >  1:2869363/1‑69 (MQ=255)
    gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat                                                 >  1:2772058/1‑69 (MQ=255)
    gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat                                                 >  1:2771509/1‑69 (MQ=255)
    gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat                                                 >  1:3415101/1‑69 (MQ=255)
    gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat                                                 >  1:359436/1‑69 (MQ=255)
    gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat                                                 >  1:743941/1‑69 (MQ=255)
    gaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGcatcat                                                 >  1:1234120/1‑69 (MQ=255)
     accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc                                                <  1:142734/69‑1 (MQ=255)
     accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc                                                <  1:2914722/69‑1 (MQ=255)
     accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc                                                <  1:3211541/69‑1 (MQ=255)
     accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc                                                <  1:2149008/69‑1 (MQ=255)
     accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc                                                <  1:911567/69‑1 (MQ=255)
     accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc                                                <  1:1752639/69‑1 (MQ=255)
     accgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCatcatc                                                <  1:1837477/69‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCGAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:679566/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:1043650/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:453661/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:1439900/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:348246/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:777245/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:801216/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:1631934/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:897121/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:1665669/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:2562807/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:2128440/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:1748749/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:2051537/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:2006058/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:2591723/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:277232/57‑1 (MQ=255)
       cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATTGGGATACCAGCCAgtga                                                          <  1:2700952/57‑1 (MQ=255)
             cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGtcat                                        <  1:1688249/69‑1 (MQ=255)
             cACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGtcat                                        <  1:225859/69‑1 (MQ=255)
                           cGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCCTCATCACCGTCATCACTGCCGCGAgg                           <  1:3239007/68‑1 (MQ=255)
                           cGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAgg                           <  1:2614607/68‑1 (MQ=255)
                            gATAAAGCCCAGCGTCATGGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAgg                           <  1:2379509/67‑1 (MQ=255)
                                  gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGAc                    <  1:594996/68‑1 (MQ=255)
                                  gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGAc                    <  1:2376528/68‑1 (MQ=255)
                                  gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGAc                    <  1:1572077/68‑1 (MQ=255)
                                          tCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAg            >  1:1920881/1‑68 (MQ=255)
                                          tCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAg            >  1:2402149/1‑68 (MQ=255)
                                          tCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAg            >  1:1447005/1‑68 (MQ=255)
                                                    aCCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACcgc  >  1:2128839/1‑68 (MQ=255)
                                                    aCCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACcgc  >  1:2472780/1‑68 (MQ=255)
                                                       |                                                                
CACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGC  >  minE/1486285‑1486404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: