| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,486,500 | G→A | 38.0% | A271V (GCC→GTC) | nuoN ← | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,486,500 | 0 | G | A | 38.0% | 47.7 / 52.7 | 84 | A271V (GCC→GTC) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base G (30/22); new base A (12/20); total (42/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACC > minE/1486439‑1486562 | aTACGGGCTGGACATCATGCTGACCTCGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:162840/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:3042800/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:44800/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:2019826/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:2118287/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:1602824/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:1542899/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:2811818/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:3141069/69‑1 (MQ=255)aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:2999054/69‑1 (MQ=255) tACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:3374419/68‑1 (MQ=255) tACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:73291/68‑1 (MQ=255) tACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:1816595/68‑1 (MQ=255) cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCtt > 1:1637704/1‑68 (MQ=255) cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:3350393/1‑69 (MQ=255) cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:329960/1‑69 (MQ=255) cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:1973631/1‑69 (MQ=255) cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:207274/1‑69 (MQ=255) cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:508934/1‑69 (MQ=255) cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:57734/1‑69 (MQ=255) cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:1374227/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCGTACCGCTTcc > 1:2545625/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:922561/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:811576/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:3229645/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:2758763/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:260481/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:2401529/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:1379639/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:660853/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:1701228/1‑69 (MQ=255) aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc < 1:2192368/68‑1 (MQ=255) cccACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2518080/2‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGa > 1:2709375/1‑68 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:873524/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2888443/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:670446/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:776909/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2702029/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:378431/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2475505/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:1407598/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:3347999/1‑69 (MQ=255) aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2191737/1‑69 (MQ=255) cACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAt < 1:1708889/69‑1 (MQ=255) cACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAt < 1:2258235/69‑1 (MQ=255) cgcgCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:535847/36‑1 (MQ=255) gcgcCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:1074107/35‑1 (MQ=255) gcttctGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgcgc < 1:2341033/69‑1 (MQ=255) gctgctGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgcgc < 1:3093528/69‑1 (MQ=255) gctgctGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgcgc < 1:3171834/69‑1 (MQ=255) ctgctgAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCa > 1:867730/1‑69 (MQ=255) gAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc < 1:1227652/36‑1 (MQ=255) agcAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:820866/65‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCTCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:461560/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:746417/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1764847/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:64387/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:630836/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:3028091/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1854006/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1883846/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1932857/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2089949/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2153639/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:851913/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2604918/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2887152/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1109322/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1015571/67‑1 (MQ=255) aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:936327/67‑1 (MQ=255) aCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1843572/66‑1 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTg > 1:853692/1‑50 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:744013/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:1736382/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:1763058/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:1154643/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:3393422/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:2299623/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:3227147/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:890582/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:3089751/1‑69 (MQ=255) tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:3088813/1‑69 (MQ=255) aCCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2917448/59‑1 (MQ=255) | ATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACC > minE/1486439‑1486562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |