| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,486,694 | G→A | 66.6% | S206S (AGC→AGT) | nuoN ← | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,486,694 | 0 | G | A | 66.6% | 68.9 / 50.6 | 94 | S206S (AGC→AGT) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base G (8/23); new base A (23/39); total (32/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
CGCCATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCG > minE/1486628‑1486748 | cgcCATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAAtcgcttcgc < 1:3274625/68‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:2269758/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:1778785/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:1997835/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:572873/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:2534684/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:1474035/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:783160/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:1179071/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:784708/69‑1 (MQ=255) ccATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:1090080/69‑1 (MQ=255) cATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGt < 1:2446702/68‑1 (MQ=255) aGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTcaccc > 1:637840/1‑69 (MQ=255) aGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTcaccc > 1:474708/1‑69 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:2402086/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:2391279/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:2567048/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:2187304/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:2350852/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:2932892/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:3052767/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:2356199/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:1796666/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:3274182/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:1177886/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:1373866/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:654045/68‑1 (MQ=255) gTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:1528746/68‑1 (MQ=255) tACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCCCCACGCGAATCGCTTCGCTGTCACCCACCg > 1:1630808/1‑69 (MQ=255) aaGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCg < 1:2203364/69‑1 (MQ=255) aaGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCg < 1:2366285/69‑1 (MQ=255) aaGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCg < 1:2653188/69‑1 (MQ=255) aaGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCg < 1:376933/69‑1 (MQ=255) gatgatGGAGGCAAAGGCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTcaccca < 1:1983953/59‑1 (MQ=39) gatgatGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTa < 1:894640/69‑1 (MQ=255) aaaGGCGATAATCGCCAGCACCACGCGAATCGCTTCTCTGTCACCCACCGGTGCGTAGAGGAACAGACg > 1:2617519/1‑69 (MQ=255) aaaGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACg < 1:15284/69‑1 (MQ=255) aaaGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACg < 1:712642/69‑1 (MQ=255) aaGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACAGGTGCGTAGAGGAACAGACg < 1:69177/68‑1 (MQ=255) gCGATATTCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:438766/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAAtcgcttcact > 1:2881043/1‑35 (MQ=25) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGt > 1:1757429/1‑37 (MQ=37) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGGACAAACGCATc > 1:981785/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCAt > 1:2325328/1‑68 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCAt > 1:662874/1‑68 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:3237005/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:738223/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:1491609/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:1497475/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:505729/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:459161/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:173632/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2400080/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:3208336/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:297999/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:29555/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:1826388/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:1937003/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2820034/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2667625/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:231331/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2522969/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2607136/1‑69 (MQ=255) gCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAgg < 1:1740785/57‑1 (MQ=255) gCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAgg < 1:547344/57‑1 (MQ=255) gATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcac < 1:1377205/69‑1 (MQ=255) aaTCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcaccac > 1:814620/1‑69 (MQ=255) aaTCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcaccac > 1:787501/1‑69 (MQ=255) aaTCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcaccac > 1:2170444/1‑69 (MQ=255) aaTCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcaccac > 1:2626965/1‑69 (MQ=255) cAGCA‑CACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATCACCACACCGaa < 1:1964516/68‑1 (MQ=255) accacGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATCACCACACCGAAGATa > 1:3127515/1‑69 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:247200/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1496257/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2435626/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2302196/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2424066/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1289592/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:115691/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:110586/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:962193/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1081768/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:600953/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1561000/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1592695/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:279011/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2216542/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:17444/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3273639/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3239046/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3207294/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3068188/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1812172/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2774316/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGAAAAGGATCAAACGCATCACCACGCCGAAGATAGCg < 1:1732979/69‑1 (MQ=255) acGCGAATCGCATCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3426052/69‑1 (MQ=255) | CGCCATCAGGTTACCGAAGATGATGGAGGCAAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCG > minE/1486628‑1486748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |