| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,486,723 | G→A | 61.9% | L197L (CTG→TTG) | nuoN ← | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,486,723 | 0 | G | A | 61.9% | 37.1 / 49.1 | 76 | L197L (CTG→TTG) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base G (8/21); new base A (22/25); total (30/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTGGAA > minE/1486658‑1486778 | aaaGGCGATAATCGCCAGCACCACGCGAATCGCTTCTCTGTCACCCACCGGTGCGTAGAGGAACAGACg > 1:2617519/1‑69 (MQ=255)aaaGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACg < 1:712642/69‑1 (MQ=255)aaaGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACg < 1:15284/69‑1 (MQ=255) aaGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACAGGTGCGTAGAGGAACAGACg < 1:69177/68‑1 (MQ=255) gCGATATTCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:438766/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGGACAAACGCATc > 1:981785/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCAt > 1:2325328/1‑68 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCAt > 1:662874/1‑68 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:1497475/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:231331/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2400080/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2522969/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2607136/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2667625/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:2820034/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:29555/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:1937003/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:1826388/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:297999/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:3208336/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:173632/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:3237005/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:459161/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:505729/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:738223/1‑69 (MQ=255) gCGATAATCGCCAGCACTACGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATc > 1:1491609/1‑69 (MQ=255) gATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcac < 1:1377205/69‑1 (MQ=255) aaTCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcaccac > 1:787501/1‑69 (MQ=255) aaTCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcaccac > 1:814620/1‑69 (MQ=255) aaTCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcaccac > 1:2626965/1‑69 (MQ=255) aaTCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATcaccac > 1:2170444/1‑69 (MQ=255) cAGCA‑CACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATCACCACACCGaa < 1:1964516/68‑1 (MQ=255) accacGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATCACCACACCGAAGATa > 1:3127515/1‑69 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:600953/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3068188/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3207294/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:17444/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2302196/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3273639/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1592695/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3239046/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1561000/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1496257/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1289592/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:115691/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:962193/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:110586/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1812172/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2424066/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2435626/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:247200/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2216542/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:1081768/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:2774316/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:279011/69‑1 (MQ=255) acGCGAATCGCTTCACTGTCACCCACCGGTGCGAAAAGGATCAAACGCATCACCACGCCGAAGATAGCg < 1:1732979/69‑1 (MQ=255) acGCGAATCGCATCACTGTCACCCACCGGTGCGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:3426052/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGt > 1:3431148/1‑68 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGt > 1:2012329/1‑68 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:2788790/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:1295097/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:2024425/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:483735/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:809528/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:1676854/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:3402853/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:3401745/69‑1 (MQ=255) ggTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:3044969/69‑1 (MQ=255) gTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:766368/68‑1 (MQ=255) gTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:2727173/68‑1 (MQ=255) gTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:2523899/68‑1 (MQ=255) gTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:1648819/68‑1 (MQ=255) gTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:3285515/68‑1 (MQ=255) tGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTg < 1:508886/67‑1 (MQ=255) gCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTGGaa < 1:1970127/69‑1 (MQ=255) cGTAAAGGAACAAACGCATCACCACGCCGAAGATAGCg < 1:112152/38‑1 (MQ=25) | AAAGGCGATAATCGCCAGCACCACGCGAATCGCTTCGCTGTCACCCACCGGTGCGTAGAGGAACAGACGCATCACCACACCGAAGATAGCGATTTTGCTCGCCGTCGCCAGGAAAGTGGAA > minE/1486658‑1486778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |