| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,488,467 | A→G | 73.5% | I187I (ATT→ATC) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,488,467 | 0 | A | G | 73.5% | 108.6 / 35.5 | 94 | I187I (ATT→ATC) | nuoM | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Reads supporting (aligned to +/- strand): ref base A (18/7); new base G (24/45); total (42/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.02e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCG > minE/1488399‑1488531 | agcTCTTCATAGTTGAAGGGCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCa < 1:473727/69‑1 (MQ=255) ctctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATTGCaa > 1:3066831/1‑68 (MQ=255) ctctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCaa > 1:886075/1‑68 (MQ=255) ctctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCaa > 1:157863/1‑68 (MQ=255) ctctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCaa > 1:2386903/1‑68 (MQ=255) ctctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCaa > 1:105241/1‑68 (MQ=255) ctctTCATAGTTGAAGGTACAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCa > 1:3248532/1‑67 (MQ=255) cATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAAc > 1:865269/1‑68 (MQ=255) tAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAAc < 1:1620597/66‑1 (MQ=255) tGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCAc < 1:1621049/67‑1 (MQ=255) ccAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCa < 1:669929/49‑1 (MQ=255) ccAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCa < 1:2504428/49‑1 (MQ=255) ccAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCa < 1:1852558/49‑1 (MQ=255) ccAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCa < 1:1661692/49‑1 (MQ=255) gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:666265/1‑68 (MQ=255) gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:1931296/1‑68 (MQ=255) gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:1831518/1‑68 (MQ=255) gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:1021568/1‑68 (MQ=255) aGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaag < 1:2225037/68‑1 (MQ=255) gTGAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACT‑‑CCTGGGTGTAAATGAAGAAc < 1:405860/68‑1 (MQ=255) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:3365258/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2858643/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2705890/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2564492/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2981725/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:386196/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:592725/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1894943/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:13136/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1410425/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:167634/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:147032/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1409243/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1588851/68‑1 (MQ=39) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt > 1:738888/1‑69 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt > 1:2726750/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGc > 1:3412963/1‑43 (MQ=37) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTgg > 1:463226/1‑47 (MQ=37) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:461440/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1156237/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2379688/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:3013666/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:869816/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1257403/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1790918/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2649396/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2888628/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2280432/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1746668/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1851817/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1948416/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:381033/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1084670/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1118372/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:6562/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:638602/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2273504/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2304703/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACAAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:730549/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:1000563/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:1287423/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:428037/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:2835546/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:2839325/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgcc > 1:936533/1‑68 (MQ=255) gATGGCGATCAACATCCCCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3104970/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1126520/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:561947/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:583025/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:682596/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:754199/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:764556/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1538960/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3389345/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3378263/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1506893/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1601037/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1427115/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1631176/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1914240/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:215467/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2255036/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2330103/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1284125/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2479723/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2569311/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2936488/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3031068/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3049248/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1186067/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3387425/55‑1 (MQ=38) gATGGCGAGCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGAtt < 1:2309748/55‑1 (MQ=37) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcgcg > 1:2250844/1‑69 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcgcg > 1:2749390/1‑69 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcgcg > 1:1972794/1‑69 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGCGATAcgcg > 1:1612076/1‑69 (MQ=255) gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:24515/61‑1 (MQ=255) gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:129519/61‑1 (MQ=255) gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:1072069/61‑1 (MQ=255) cAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:2264489/60‑1 (MQ=255) | AGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCG > minE/1488399‑1488531 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |