| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,488,482 | G→A | 63.7% | G182G (GGC→GGT) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,488,482 | 0 | G | A | 63.7% | 89.8 / 92.7 | 146 | G182G (GGC→GGT) | nuoM | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Reads supporting (aligned to +/- strand): ref base G (31/22); new base A (48/45); total (79/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
GTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTT > minE/1488437‑1488549 | gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:1021568/1‑68 (MQ=255)gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:1831518/1‑68 (MQ=255)gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:414919/1‑68 (MQ=255) aGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaag < 1:2225037/68‑1 (MQ=255) gTGAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACT‑‑CCTGGGTGTAAATGAAGAAc < 1:405860/68‑1 (MQ=255) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:13136/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1894943/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:592725/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:147032/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1410425/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1409243/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2564492/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2858643/68‑1 (MQ=39) gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2981725/68‑1 (MQ=39) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt > 1:738888/1‑69 (MQ=255) aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt > 1:2726750/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTgg > 1:463226/1‑47 (MQ=37) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2273504/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:3013666/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2304703/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2379688/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:381033/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1851817/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1790918/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:6562/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1084670/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1746668/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCCCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1562615/1‑69 (MQ=255) aaaCCAGCGCCAGGATGGCGATCAACATCACAAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:730549/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:1287423/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:1000563/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:2835546/1‑69 (MQ=255) ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgcc > 1:936533/1‑68 (MQ=255) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3389345/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2801593/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1126520/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3378263/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1186067/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2479723/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3031068/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2936488/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:764556/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2255036/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1631176/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1506893/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1601037/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1914240/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:682596/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:561947/55‑1 (MQ=38) gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:215467/55‑1 (MQ=38) gATGGCGAGCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGAtt < 1:2309748/55‑1 (MQ=37) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcgcg > 1:2749390/1‑69 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcgcg > 1:1972794/1‑69 (MQ=255) tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGCGATAcgcg > 1:1612076/1‑69 (MQ=255) gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:129519/61‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc < 1:541461/69‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc < 1:1940732/69‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc < 1:2542963/69‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc < 1:806771/69‑1 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGGCGTGATACGCGt > 1:867784/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:356774/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:496623/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:3249244/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:266813/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:120453/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2102240/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:1960801/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2200817/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:1230529/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2342133/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2601122/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2879555/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2898114/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:1247674/1‑64 (MQ=255) tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:1704808/1‑64 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:2835625/1‑69 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:3400974/1‑69 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:912336/1‑69 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc < 1:3263674/69‑1 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:845129/1‑69 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:820578/1‑69 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:2195086/1‑69 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:937421/1‑69 (MQ=255) cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGATTTAcc > 1:1263246/1‑69 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:2920091/68‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:1107659/68‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:655450/68‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:1562238/68‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTAGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:966231/68‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:2127005/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1443244/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:6074/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:439724/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:439464/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:225216/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:2697603/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:3428985/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:3171229/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:3096052/69‑1 (MQ=255) ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:3069322/69‑1 (MQ=255) | GTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTT > minE/1488437‑1488549 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |