| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,489,398 | A→G | 100% | Y545Y (TAT→TAC) | nuoL ← | NADH:ubiquinone oxidoreductase, membrane subunit L |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,489,398 | 0 | A | G | 77.9% | 45.0 / 8.5 | 36 | Y545Y (TAT→TAC) | nuoL | NADH:ubiquinone oxidoreductase, membrane subunit L |
| Reads supporting (aligned to +/- strand): ref base A (0/8); new base G (19/9); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.03e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGC > minE/1489332‑1489466 | tGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCAt < 1:2934272/68‑1 (MQ=255)tGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCAt < 1:1849073/68‑1 (MQ=255)tGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCAt < 1:2652996/68‑1 (MQ=255)tGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCAt < 1:2540482/68‑1 (MQ=255)tGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCAt < 1:770548/68‑1 (MQ=255) tCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGcc < 1:195361/69‑1 (MQ=255) cAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGcc < 1:1348585/68‑1 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCa > 1:3154870/1‑65 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:593957/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:348130/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:710791/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:336304/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:766871/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:2717257/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:2704502/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:2589998/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:2473164/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:2244493/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:815172/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:1991072/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:191426/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:1909529/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:1793406/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:1647925/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:1577902/1‑67 (MQ=255) ggATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGt > 1:1501830/1‑67 (MQ=255) cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTAGAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:2736385/69‑1 (MQ=255) cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:826679/69‑1 (MQ=255) cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:1254922/69‑1 (MQ=255) cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:945168/69‑1 (MQ=255) cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:3402798/69‑1 (MQ=255) cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:23020/69‑1 (MQ=255) cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:2051408/69‑1 (MQ=255) cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:1276519/69‑1 (MQ=255) aaCACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:3324798/42‑1 (MQ=37) cacTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGgcgc < 1:174886/69‑1 (MQ=255) aTACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGc < 1:538903/69‑1 (MQ=255) | TGAGTTCAGCGGATCGCGTTTCAGCAACCAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGC > minE/1489332‑1489466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |