| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,489,413 | T→G | 100% | G540G (GGA→GGC) | nuoL ← | NADH:ubiquinone oxidoreductase, membrane subunit L |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,489,413 | 0 | T | G | 88.3% | 73.2 / 1.2 | 34 | G540G (GGA→GGC) | nuoL | NADH:ubiquinone oxidoreductase, membrane subunit L |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base G (21/9); total (23/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
CAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTC > minE/1489360‑1489476 | cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTAGAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:2736385/69‑1 (MQ=255)cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:945168/69‑1 (MQ=255)cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:1254922/69‑1 (MQ=255)cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:1276519/69‑1 (MQ=255)cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:826679/69‑1 (MQ=255)cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:23020/69‑1 (MQ=255)cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:2051408/69‑1 (MQ=255)cAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:3402798/69‑1 (MQ=255) aaCACTTTGTCGTACAGCCAGTCAAAGCCCCAGGCGTTGTac < 1:3324798/42‑1 (MQ=37) cacTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGgcgc < 1:174886/69‑1 (MQ=255) aTACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGc < 1:538903/69‑1 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGc > 1:3344054/1‑45 (MQ=38) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGgcg > 1:1820847/1‑51 (MQ=38) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:1247887/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:3416153/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:3295895/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:695312/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:3276397/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:752909/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:3132777/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:293778/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:281163/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:2740836/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:2157803/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:2068864/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:1996342/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:1946992/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:1691628/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:1599201/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:1524786/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:1070969/1‑68 (MQ=255) aGTCAAAGCCCCAGGCGTTGTACCACAAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg > 1:2439203/1‑68 (MQ=255) cAACTCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTc > 1:1786059/1‑68 (MQ=255) cAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTc > 1:1619654/1‑68 (MQ=255) | CAGGCAATACCCAGGAACGGCTTGACGAACACTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTC > minE/1489360‑1489476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |