Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,489,437 G→A 100% G532G (GGC→GGT)  nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,489,4370GA84.1% 36.2 / 0.6 25G532G (GGC→GGT) nuoLNADH:ubiquinone oxidoreductase, membrane subunit L
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (21/0);  total (23/2)
Fisher's exact test for biased strand distribution p-value = 2.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTCACCAGAGTACGTTTACCCAGCCACAGCCA  >  minE/1489389‑1489505
                                                |                                                                    
cacTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGgcgc                                                  <  1:174886/69‑1 (MQ=255)
         aTACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGc                                         <  1:538903/69‑1 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGc                                                         >  1:3344054/1‑45 (MQ=38)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGgcg                                                   >  1:1820847/1‑51 (MQ=38)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:2157803/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:752909/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:695312/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:3416153/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:3295895/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:3276397/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:3132777/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:293778/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:281163/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:2740836/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:1070969/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:2068864/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:1996342/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:1946992/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:1691628/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:1599201/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:1524786/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACCAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:1247887/1‑68 (MQ=255)
                 aGTCAAAGCCCCAGGCGTTGTACCACAAGGTACCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAg                                  >  1:2439203/1‑68 (MQ=255)
                    cAACTCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTc                               >  1:1786059/1‑68 (MQ=255)
                    cAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTc                               >  1:1619654/1‑68 (MQ=255)
                                                gCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTCACCAGAGTACGTTTACCcagccacagcca  >  1:2842920/1‑69 (MQ=255)
                                                gCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTCACCAGAGTACGTTTACCcagccacagcca  >  1:1205051/1‑69 (MQ=255)
                                                gCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTCACCAGAGTACGTTTACCcagccacagcc   >  1:1075292/1‑68 (MQ=255)
                                                |                                                                    
CACTTTGTCATACAGCCAGTCAAATCCCCAGGCGTTGTACCACCAGGTGCCCAGCAGACGGCCCGGCGCACTGTTGGCGATGGAGGTCACCAGAGTACGTTTACCCAGCCACAGCCA  >  minE/1489389‑1489505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑