| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,492,939 | A→G | 50.8% | L164L (TTG→CTG) | nuoH ← | NADH:ubiquinone oxidoreductase, membrane subunit H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,492,939 | 0 | A | G | 50.8% | ‑2.7 / 53.7 | 61 | L164L (TTG→CTG) | nuoH | NADH:ubiquinone oxidoreductase, membrane subunit H |
| Reads supporting (aligned to +/- strand): ref base A (26/4); new base G (13/18); total (39/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
ATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAG > minE/1492874‑1493006 | aTGCGCCTGGCTGTTGACGATGTCGGTCATGTT‑AATGAACCGGCCT‑CGCCACCACGCCCATCAGGGaaa < 1:127607/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1104069/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:756753/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:459127/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:447202/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:2760770/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:2645774/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:2217639/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1899355/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1768387/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1477840/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1358760/69‑1 (MQ=255) gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1204414/69‑1 (MQ=255) cgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1546384/68‑1 (MQ=255) cgcCTGGCTGTTGACGATGTCAGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAg < 1:1364951/69‑1 (MQ=255) gcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1198836/67‑1 (MQ=255) ccTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:233800/66‑1 (MQ=255) gTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:2751701/59‑1 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:3382885/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:2779067/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:3173269/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:2398742/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:2239756/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:1141370/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:559265/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:1068401/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:909854/1‑69 (MQ=255) gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAacac > 1:1435499/1‑69 (MQ=255) cGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGaaa < 1:1642008/54‑1 (MQ=255) tCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAg > 1:2275527/1‑69 (MQ=255) tCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAg > 1:1285207/1‑69 (MQ=255) tCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAg > 1:651434/1‑69 (MQ=255) cATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAg > 1:2363741/1‑69 (MQ=255) cATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAg > 1:3075943/1‑69 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:96464/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:3318524/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:3310308/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:3277299/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:2572541/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:1959475/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:2561877/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:2284996/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:2533127/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:2416452/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:2503369/1‑65 (MQ=255) aTGTTGAATGAACCGGCCTGCGACACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:2758540/1‑65 (MQ=255) aTGTTGAATGAACCGGACTGCGCCACCACGCCCATCAGGGAAAGCCCGAGGAACACTTCGTAGCt > 1:789388/1‑65 (MQ=255) acGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCAccc > 1:2054496/1‑69 (MQ=255) acGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCAccc > 1:546617/1‑69 (MQ=255) acGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCAccc > 1:698851/1‑69 (MQ=255) acGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCAccc > 1:3112439/1‑69 (MQ=255) acGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCAccc > 1:2975107/1‑69 (MQ=255) cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTgcgc > 1:871093/1‑47 (MQ=255) cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTgcgc > 1:466042/1‑47 (MQ=255) cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTgcgc > 1:2231905/1‑47 (MQ=255) cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCAca > 1:622895/1‑55 (MQ=255) cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACg > 1:768869/1‑56 (MQ=255) cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACg > 1:1444612/1‑56 (MQ=255) cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGc < 1:1204671/69‑1 (MQ=255) ccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGc < 1:2582290/68‑1 (MQ=255) ccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGc < 1:2432977/68‑1 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCAt > 1:931459/1‑53 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:701919/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg < 1:2537578/69‑1 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg < 1:149751/69‑1 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:2863698/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:820330/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:2867432/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:3096963/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg < 1:1618380/69‑1 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:755209/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:1715236/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:626097/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg < 1:3221334/69‑1 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:1244126/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:3243204/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:3315868/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:335548/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg > 1:451995/1‑69 (MQ=255) aaGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACCCATCGCACCCAGCAACGAg < 1:784936/69‑1 (MQ=255) aGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAg < 1:1234071/68‑1 (MQ=255) | ATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAG > minE/1492874‑1493006 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |