| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,493,345 | G→A | 60.8% | C28C (TGC→TGT) | nuoH ← | NADH:ubiquinone oxidoreductase, membrane subunit H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,493,345 | 0 | G | A | 60.8% | 25.9 / 42.1 | 65 | C28C (TGC→TGT) | nuoH | NADH:ubiquinone oxidoreductase, membrane subunit H |
| Reads supporting (aligned to +/- strand): ref base G (19/6); new base A (19/20); total (39/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
TAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATC > minE/1493281‑1493405 | tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:439410/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1850053/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3250976/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3332184/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1557010/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:2415172/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3404406/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3209761/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:2159548/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:371625/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:405278/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1356532/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1345456/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:2180312/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:622235/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1260344/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3207521/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1188458/69‑1 (MQ=255)tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:623959/69‑1 (MQ=255) ggTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1034254/67‑1 (MQ=255) cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACa > 1:3088021/1‑68 (MQ=255) cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACa > 1:3021693/1‑68 (MQ=255) cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACa > 1:585965/1‑68 (MQ=255) aCAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATc < 1:2752715/68‑1 (MQ=255) aGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCTCAGGTGACTACCACCAGCAGGATcacc > 1:1408321/1‑69 (MQ=255) aGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGATcac > 1:123335/1‑68 (MQ=255) gACCGAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacc > 1:192237/1‑68 (MQ=255) gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca > 1:2619109/1‑69 (MQ=255) gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca > 1:2274474/1‑69 (MQ=255) gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca > 1:3220392/1‑69 (MQ=255) gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca > 1:3393010/1‑69 (MQ=255) gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca > 1:1419607/1‑69 (MQ=255) gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca > 1:946171/1‑69 (MQ=255) gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacc > 1:2547687/1‑68 (MQ=255) gACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacc > 1:137688/1‑68 (MQ=255) gCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAg < 1:2574812/69‑1 (MQ=255) gCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAg < 1:181164/69‑1 (MQ=255) gCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAg < 1:2125007/69‑1 (MQ=255) gCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAg < 1:819967/69‑1 (MQ=255) gCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAg < 1:820736/69‑1 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc > 1:797119/1‑47 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc > 1:795623/1‑47 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc > 1:2781924/1‑47 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc > 1:1549715/1‑47 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcag > 1:1724979/1‑46 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGa > 1:2079335/1‑36 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGCCTACCACCCGCAGGAt > 1:1169995/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGa > 1:476441/1‑51 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:1266717/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:13697/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:418905/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:1376537/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:1089855/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:3436203/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:1467162/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:2182255/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:2240881/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:3054563/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:2441772/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:289253/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:2852964/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:2487604/1‑52 (MQ=255) gACGTTCGCCAAAGCTCATGAATGCCCCACAGGTGACTACCACCAGCAGGAt > 1:2641380/1‑52 (MQ=255) aaTGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAAt > 1:3303772/1‑68 (MQ=255) aaTGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATc > 1:1265680/1‑69 (MQ=255) | TAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATC > minE/1493281‑1493405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |