| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,495,138 | G→A | 100% | Y340Y (TAC→TAT) | nuoG ← | NADH:ubiquinone oxidoreductase, chain G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,495,138 | 0 | G | A | 75.4% | 101.7 / 29.3 | 85 | Y340Y (TAC→TAT) | nuoG | NADH:ubiquinone oxidoreductase, chain G |
| Reads supporting (aligned to +/- strand): ref base G (14/7); new base A (64/0); total (78/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCGCCTTCACGCAGCACTTTCAGCGCCAGTTGCAGACGTTCCTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCTGGCACGCGGAGAACC > minE/1495072‑1495206 | gccgccTTCACGCAGCACTTTCAGCGCCAGTTGCAGACGTTCCTGCTCACCGTGAGCGATACCGgtgt > 1:770346/1‑68 (MQ=255)gccgccTTCACGCAGCACTTTCAGCGCCAGTTGCAGACGTTCCTGCTCACCGTGAGCGATACCGgtgt > 1:3242243/1‑68 (MQ=255) cGCAGCCCTTTCAGCGCCAGTTGCAGACGTTCCTGCTCACCGTGAGCGATACCGGTGTAGAAGTTttc < 1:2242519/68‑1 (MQ=255) gCAGACGTTCCTGCTCACCGTGAGCGATAACGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAgc > 1:589767/1‑68 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGc > 1:2098821/1‑61 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:2509465/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:1425100/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:3000281/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:2974095/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:2885425/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:2837502/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:2519423/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:3050302/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:1538289/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:1561893/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:1968604/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:19651/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:301050/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:3213899/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:1321420/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:3328747/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:455208/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:628388/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:646710/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:700134/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:744786/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:1072089/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:857187/1‑69 (MQ=255) aCGTTCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCaaa > 1:1041640/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGttt > 1:2444482/1‑36 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCGGCGCAAAGTTg > 1:1872214/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:2901704/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:1445881/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:1513067/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:2783202/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:2985284/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:2721862/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:2678937/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:3196171/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:3262947/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:1531458/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:2416672/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:2365091/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:3314966/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:1293956/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:3374158/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:1550571/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:619024/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:751317/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:805549/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:1853741/1‑69 (MQ=255) tCCTGCTCACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:1930119/1‑69 (MQ=255) tCCTGCACACCGTGAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTg > 1:3400739/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTg > 1:851177/1‑68 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:3309087/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:1114612/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:3133836/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:964943/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:2863981/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:1631740/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:213519/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:2046642/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:2050996/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:2692062/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:2319363/1‑69 (MQ=255) ccTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGc > 1:1039110/1‑69 (MQ=255) gTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCt < 1:662076/69‑1 (MQ=255) gTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCt < 1:1286852/69‑1 (MQ=255) gTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCt < 1:1488355/69‑1 (MQ=255) gTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCt < 1:626954/69‑1 (MQ=255) gTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCt < 1:1042600/69‑1 (MQ=255) gTGAGCGATACCGCTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCt < 1:1968042/69‑1 (MQ=255) gAGCTATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:1074494/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGc > 1:1297586/1‑57 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:1253291/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:1542773/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:1176601/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:2057297/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:514780/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:1290133/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:251432/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:1522871/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:139959/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:1417831/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:276918/1‑59 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCGCCCACCAGCTCACGCAGCGCAAAGTTGCt > 1:3312229/1‑58 (MQ=255) gAGCGATACCGGTATAGAAGTTTTCTTCCCCCACCAGCTCACGCAGCGCAAAGTTGCtt > 1:1254009/1‑59 (MQ=255) tgtAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCTGG‑ACGCGGAGAAcc < 1:1392895/69‑1 (MQ=255) gtAGAAGTTTTCTTCGCCCACCAGTTGACGCAGCGCAAAGTTGCTTTCCACGCTGGCACGCGGAGAAcc < 1:1437217/69‑1 (MQ=255) gtAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCTGGCACGCGGAGAAcc < 1:2225133/69‑1 (MQ=255) gtAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCTGGCACGCGGAGAAcc < 1:1928364/69‑1 (MQ=255) gtAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCTGGCACGCGGAGAAcc < 1:2997108/69‑1 (MQ=255) gtAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCTGGCACGCGGAGAAcc < 1:2953347/69‑1 (MQ=255) | GCCGCCTTCACGCAGCACTTTCAGCGCCAGTTGCAGACGTTCCTGCTCACCGTGAGCGATACCGGTGTAGAAGTTTTCTTCGCCCACCAGTTCACGCAGCGCAAAGTTGCTTTCCACGCTGGCACGCGGAGAACC > minE/1495072‑1495206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |