| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,497,661 | A→G | 49.5% | F126F (TTT→TTC) | nuoE ← | NADH:ubiquinone oxidoreductase, chain E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,497,661 | 0 | A | G | 49.5% | ‑5.9 / 88.8 | 95 | F126F (TTT→TTC) | nuoE | NADH:ubiquinone oxidoreductase, chain E |
| Reads supporting (aligned to +/- strand): ref base A (25/23); new base G (22/25); total (47/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
TCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACC > minE/1497602‑1497729 | tCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGc < 1:3118108/67‑1 (MQ=255) catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTaa > 1:3093417/1‑56 (MQ=255) catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaaa > 1:1590477/1‑69 (MQ=255) catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaaa > 1:967532/1‑69 (MQ=255) catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaaa > 1:261903/1‑69 (MQ=255) catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaaa > 1:189207/1‑69 (MQ=255) catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaaa > 1:878309/1‑69 (MQ=255) catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaaa > 1:1970548/1‑69 (MQ=255) catcatGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaa > 1:684912/1‑68 (MQ=255) cacaGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTg < 1:1685023/68‑1 (MQ=255) cacaGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTg < 1:540062/68‑1 (MQ=255) cacaGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTg < 1:320884/68‑1 (MQ=255) cacaGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTg < 1:2432143/68‑1 (MQ=255) acaGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGatttc > 1:2181581/1‑66 (MQ=255) acaGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGatttc > 1:2848761/1‑66 (MQ=255) tCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCa > 1:2732552/1‑69 (MQ=255) tCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTATGCCCTGGTTTGATGTTc > 1:787459/1‑68 (MQ=255) ccccAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCa > 1:2204880/1‑68 (MQ=255) ccccAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAg > 1:2562124/1‑69 (MQ=255) ccccAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAg > 1:600004/1‑69 (MQ=255) ccccAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAg > 1:1147377/1‑69 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:3229037/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:1094676/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2431471/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:3349765/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2641393/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:850382/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:1456516/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2180161/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:406970/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2148497/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2804792/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:448603/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:3236985/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:767449/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:727351/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:490298/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2839287/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2889864/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:1488758/68‑3 (MQ=255) cAGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:677137/68‑3 (MQ=255) aGGCAGCAAGTTGGCAGCAGCGTGAAGCGACCGGCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:3341713/67‑3 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:2904740/68‑1 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:297614/68‑1 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:1072642/68‑1 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:3379001/68‑1 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:3397819/68‑1 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:935599/68‑1 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:1361994/68‑1 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:2048088/68‑1 (MQ=255) cagcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt < 1:1486282/68‑1 (MQ=255) agcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCGCTGGTTTGATGTTCAGCTTTTtc > 1:3110327/1‑68 (MQ=255) agcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCttttt > 1:1805057/1‑67 (MQ=255) agcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtc > 1:1989886/1‑68 (MQ=255) agcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtc > 1:2187861/1‑68 (MQ=255) agcaAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtc > 1:290515/1‑68 (MQ=255) agcaAGTTGGAAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtc > 1:517591/1‑68 (MQ=255) aaGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTg < 1:1849653/50‑1 (MQ=255) tGGCAGCAGCGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:925892/56‑3 (MQ=255) gcagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCgccgcc > 1:3275549/1‑68 (MQ=255) gcagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCgccgcc > 1:971651/1‑68 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2583933/50‑3 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGtttttt > 1:975358/1‑54 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGtttttt > 1:1424356/1‑54 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGtttttt > 1:1996598/1‑54 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTtttt > 1:858978/1‑53 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:671304/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:1090874/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:1140323/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:1296784/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:131063/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:921597/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:866241/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:14375/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:1462393/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:147423/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:1475128/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:1543217/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:3101697/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:185666/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:2887291/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:2679694/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:1887750/1‑55 (MQ=255) cagcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAGTTTTTtc > 1:2173791/1‑55 (MQ=255) cagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCTCCGCCTg < 1:1814104/69‑1 (MQ=255) cagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTg < 1:3144283/69‑1 (MQ=255) cagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTg < 1:2215189/69‑1 (MQ=255) cagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTg < 1:3402343/69‑1 (MQ=255) cagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTg < 1:780585/69‑1 (MQ=255) cagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTg < 1:1884037/69‑1 (MQ=255) cagcGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTg < 1:52228/69‑1 (MQ=255) agcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:1141257/49‑3 (MQ=255) gcGTGAAGCGACCGTCAAATGTCGTTTGCCCTGGTTTGATGTTCAgtt < 1:2606248/48‑3 (MQ=255) gTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATa > 1:220777/1‑69 (MQ=255) gTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATa > 1:2226820/1‑69 (MQ=255) aaaGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc < 1:912095/69‑1 (MQ=255) aaaGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc < 1:2824926/69‑1 (MQ=255) aaaGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc < 1:1645941/69‑1 (MQ=255) aaaGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc < 1:963642/69‑1 (MQ=255) aaaGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc < 1:3052137/69‑1 (MQ=255) | TCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACC > minE/1497602‑1497729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |