| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,500,204 | A→G | 39.9% | S132S (TCT→TCC) | nuoB ← | NADH:ubiquinone oxidoreductase, chain B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,500,204 | 0 | A | G | 39.9% | 37.4 / 60.1 | 89 | S132S (TCT→TCC) | nuoB | NADH:ubiquinone oxidoreductase, chain B |
| Reads supporting (aligned to +/- strand): ref base A (22/31); new base G (21/14); total (44/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
TATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGAC > minE/1500137‑1500265 | tataCACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCag < 1:775015/69‑1 (MQ=255)tataCACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCag < 1:2695696/69‑1 (MQ=255) cacaTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGtt > 1:811938/1‑69 (MQ=255) cacaTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGtt > 1:11679/1‑69 (MQ=255) cacaTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGtt > 1:543947/1‑69 (MQ=255) cacaTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGtt > 1:825548/1‑69 (MQ=255) cacaTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGtt > 1:1690900/1‑69 (MQ=255) cacaTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGtt > 1:1561559/1‑69 (MQ=255) cacaACAACCGGGATGAATTTATCGACGCCATGCACAACGGAATAAATATCGTACATACCACCAGAGtt > 1:2031219/1‑69 (MQ=255) caTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg < 1:2485236/69‑1 (MQ=255) caTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg < 1:3419532/69‑1 (MQ=255) caTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg < 1:2097608/69‑1 (MQ=255) aTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGc < 1:213683/69‑1 (MQ=255) aTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGc < 1:903588/69‑1 (MQ=255) tCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg > 1:1498545/1‑67 (MQ=255) tCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg > 1:2673453/1‑67 (MQ=255) tCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg > 1:1697984/1‑67 (MQ=255) tCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg > 1:174866/1‑67 (MQ=255) tCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg > 1:2599284/1‑67 (MQ=255) tCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg > 1:927608/1‑67 (MQ=255) tCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTg > 1:2389363/1‑66 (MQ=255) aaCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGcaca > 1:1198217/1‑69 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAGATATCGTACATACCGCCGGAGTTGGcaca > 1:3371597/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCTGAGTTGGcaca > 1:1671645/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:1146993/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:1490230/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:579264/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:2385277/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:877840/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:3269581/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:2650202/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:2665548/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:1287482/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:2180922/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:2155061/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:895375/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:1360806/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:1387289/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:702060/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:719795/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:1515003/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:996428/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:1682109/1‑68 (MQ=255) aCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGcaca > 1:1435288/1‑68 (MQ=255) cGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTgg > 1:362875/1‑62 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGTCAcc < 1:3296583/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2906390/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:291113/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:1103971/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:292435/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:3018034/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:1471457/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:410024/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:471304/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:661863/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2747413/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2716665/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2664125/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2654355/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2600450/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2327899/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2568355/69‑1 (MQ=255) gATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:890208/69‑1 (MQ=255) aTGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:1892969/68‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:994677/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:2802371/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:2023531/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:2102693/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:590756/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:233340/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:2387159/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:3432218/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:1235817/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:127144/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:2620625/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:2635908/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:31758/69‑1 (MQ=255) cccTGCACAACGGAATAAATATCGTACATACCGCCGGAGTTGGCACAGGCACCCATTGAGATAACCCAt < 1:3006329/69‑1 (MQ=255) gCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTg > 1:999636/1‑69 (MQ=255) gCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTg > 1:599791/1‑69 (MQ=255) gCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTg > 1:3412331/1‑69 (MQ=255) gCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTg > 1:2103186/1‑69 (MQ=255) gAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAcc < 1:2570191/41‑1 (MQ=255) tatCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGt < 1:2264010/69‑1 (MQ=255) tCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCa < 1:1393710/69‑1 (MQ=255) tACCACCAGAGTTGTCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGAc < 1:788980/69‑1 (MQ=255) tACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGa < 1:2512069/68‑1 (MQ=255) tACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGAc > 1:8499/1‑69 (MQ=255) tACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGAc < 1:2042890/69‑1 (MQ=255) tACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGAc < 1:1887014/69‑1 (MQ=255) tACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGAc > 1:111806/1‑69 (MQ=255) | TATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGAC > minE/1500137‑1500265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |