Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,506,598 A→G 68.5% D247D (GAT→GAC)  yfbS ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,506,5980AG68.5% 91.1 / 46.9 98D247D (GAT→GAC) yfbSpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (17/14);  new base G (45/22);  total (62/36)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 101 total aligned reads displayed.

AGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCG  >  minE/1506546‑1506666
                                                    |                                                                    
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                                      <  1:2450911/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                                      <  1:1944691/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                                      <  1:3325288/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                                      <  1:1353016/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                                      <  1:2846184/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                                      <  1:1562906/69‑1 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACTTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:2404824/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTg                                                           >  1:94951/1‑63 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGCCCAAt                                                     >  1:1492457/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCaa                                                      >  1:1265655/1‑68 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:1902827/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:2546606/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:1996005/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:201513/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:411442/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:3224354/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:345296/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:22446/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:3429928/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:2403023/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:3355321/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:2589399/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:246128/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:687173/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:891384/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:3018104/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:3059620/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:818378/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:814195/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:770109/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:757971/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:895347/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:1664365/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:624061/1‑69 (MQ=255)
 gCGTTCAACACCGATGACGTTAGCACCATAACGCTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:2421059/1‑69 (MQ=255)
 gCGTTCAACACAGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt                                                     >  1:1746644/1‑69 (MQ=255)
          acCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGa                                            <  1:134659/69‑1 (MQ=255)
             gATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc                                         <  1:1590121/69‑1 (MQ=255)
              aTGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc                                         <  1:626901/68‑1 (MQ=255)
                gACGTTTGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCgg                                       >  1:441444/1‑68 (MQ=255)
                gACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa                                                      <  1:1243018/53‑1 (MQ=255)
                gACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCggg                                      >  1:2054223/1‑69 (MQ=255)
                gACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCggg                                      >  1:597353/1‑69 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:3046915/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:2928920/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:2924991/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:2425349/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:3380156/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:236819/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:2084769/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:2052085/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:526215/69‑1 (MQ=255)
                     tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa                                 <  1:1138439/69‑1 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCg                                             >  1:86768/1‑52 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:3372213/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:3336402/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:3269526/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:314851/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:681165/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:1022097/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:1000889/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:2946549/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:1228404/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:277893/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:1377213/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:2678373/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:1897469/1‑66 (MQ=255)
                          ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa                               >  1:1482271/1‑66 (MQ=255)
                           cATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa                           <  1:428290/69‑1 (MQ=255)
                           cATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa                           <  1:458030/69‑1 (MQ=255)
                           cATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa                           <  1:10652/69‑1 (MQ=255)
                            aTAACGCTCACGTAATTTGAGATCATCCAGCCTTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa                           <  1:741905/68‑1 (MQ=255)
                               aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAc                        >  1:1483738/1‑68 (MQ=255)
                               aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg                       >  1:1701211/1‑69 (MQ=255)
                               aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg                       >  1:801023/1‑69 (MQ=255)
                               aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg                       >  1:1218260/1‑69 (MQ=255)
                               aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg                       >  1:450307/1‑69 (MQ=255)
                               aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg                       >  1:2832073/1‑69 (MQ=255)
                               aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg                       >  1:2418544/1‑69 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGTGGCGAATAGCCAGACGTCGCGCa                <  1:3386470/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:394952/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:103334/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:31519/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:1615824/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:3396368/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:1774913/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:586569/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:2125490/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:379499/68‑1 (MQ=255)
                                       gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa                <  1:1954198/68‑1 (MQ=255)
                                             tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCTCACg              >  1:2349431/1‑64 (MQ=255)
                                             tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACg              >  1:1875655/1‑64 (MQ=255)
                                             tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACg              >  1:1447067/1‑64 (MQ=255)
                                             tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACg              >  1:2671132/1‑64 (MQ=255)
                                             tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACg              >  1:2718964/1‑64 (MQ=255)
                                             tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACg              >  1:2072737/1‑64 (MQ=255)
                                             tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCg          >  1:2593758/1‑68 (MQ=255)
                                                    atcCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCg  <  1:823289/69‑1 (MQ=255)
                                                    atcCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCg  <  1:3204901/69‑1 (MQ=255)
                                                    atcCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCg  <  1:2877431/69‑1 (MQ=255)
                                                    |                                                                    
AGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCG  >  minE/1506546‑1506666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑