| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,508,705 | G→A | 36.1% | R138R (CGC→CGT) | yfbV ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,508,705 | 0 | G | A | 36.1% | 70.2 / 63.1 | 105 | R138R (CGC→CGT) | yfbV | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (48/19); new base A (17/21); total (65/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
TGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAG > minE/1508645‑1508772 | tGCGTAATCAGGGGTCAATTACAAATCATCAAGTAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:2298807/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:2350448/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:2055149/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:782189/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:1977384/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:3305905/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:381996/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:388203/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:1512906/68‑1 (MQ=255)tGCGTAATCAGGGGTCAATTACAAATAATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:3252171/68‑1 (MQ=255)tGCGTAATCAGGGGGCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:2508854/68‑1 (MQ=255) tAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:1035828/69‑1 (MQ=255) tAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:224341/69‑1 (MQ=255) tAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:2040799/69‑1 (MQ=255) tAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:1744217/69‑1 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtgt > 1:3258258/1‑68 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:452470/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:1712889/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:2281860/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:536764/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:1798632/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:3356381/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:585920/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:1833124/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:1319526/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:2932853/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:777908/1‑69 (MQ=255) aTCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:2005237/1‑69 (MQ=255) aGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAg > 1:644362/1‑68 (MQ=255) aGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAg > 1:3252125/1‑68 (MQ=255) gggTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt < 1:119466/68‑1 (MQ=255) gggTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt < 1:3244401/68‑1 (MQ=255) gggTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt < 1:3141817/68‑1 (MQ=255) gggTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt < 1:1130234/68‑1 (MQ=255) gggTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt < 1:851963/68‑1 (MQ=255) gggTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt < 1:526203/68‑1 (MQ=255) ggTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt < 1:1189650/67‑1 (MQ=255) gTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCACGCTTaa < 1:2017022/55‑1 (MQ=255) aaTTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGcc > 1:451006/1‑69 (MQ=255) aaTTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGcc > 1:971368/1‑69 (MQ=255) aaTTAAAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGATaa > 1:1227938/1‑65 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:2266805/54‑1 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:869620/54‑1 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:806426/54‑1 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:1211071/54‑1 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtg < 1:2673120/54‑1 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgt > 1:3272309/1‑53 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgt > 1:477251/1‑53 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgt > 1:39622/1‑53 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgt > 1:3039723/1‑53 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgt > 1:2334822/1‑53 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgt > 1:2350527/1‑53 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt > 1:2607860/1‑60 (MQ=255) tACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCt > 1:1285287/1‑60 (MQ=255) tACAAATCATAAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgt > 1:1813861/1‑53 (MQ=255) aCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGTGTGTCAGCTAATGCCTGGt > 1:2507082/1‑69 (MQ=255) aCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGt > 1:1851314/1‑69 (MQ=255) aCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGt > 1:3290408/1‑69 (MQ=255) aCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGt > 1:1818814/1‑69 (MQ=255) aCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGt > 1:2095073/1‑69 (MQ=255) aCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGt > 1:1401100/1‑69 (MQ=255) aCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGt > 1:2190037/1‑69 (MQ=255) aCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGACTGGt > 1:2490347/1‑69 (MQ=255) tcatcaAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTGATGCCTGGTAATCa < 1:1422306/69‑1 (MQ=255) aaaGTTTTATCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt < 1:527524/69‑1 (MQ=255) aaaGTTTTATCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt < 1:474501/69‑1 (MQ=255) aaaGTTTTATCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt < 1:540897/69‑1 (MQ=255) aaaGTTTTATCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt < 1:2241934/69‑1 (MQ=255) aaaGTTTTATCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt < 1:1018938/69‑1 (MQ=255) aaaGTTTTATCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt < 1:2174067/69‑1 (MQ=255) aaaGTTTTATCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt < 1:2723558/69‑1 (MQ=255) aaGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt > 1:2322105/1‑68 (MQ=255) aaGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt > 1:2869118/1‑68 (MQ=255) aaGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCt > 1:885288/1‑68 (MQ=255) ttATCCAGTTGTTTGAATGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACg > 1:665026/1‑69 (MQ=255) ttATCCAGTTGTTTGAAGGCGTGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACg > 1:654676/1‑69 (MQ=255) ttATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTc > 1:84620/1‑35 (MQ=255) ttATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACg > 1:2733521/1‑69 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAg > 1:3408140/1‑53 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGc > 1:3136486/1‑55 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:222734/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:2625075/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:2720958/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:1119764/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:1352046/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:1827667/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:2797252/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:2340906/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:1472047/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:2193021/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:1514843/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:363990/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:3322585/1‑56 (MQ=255) aTCCAGTTGTTTGAAGGCACGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCt > 1:2257875/1‑56 (MQ=255) cgcgcTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAg < 1:1458760/69‑1 (MQ=255) gcgcTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTc < 1:3067801/45‑1 (MQ=255) gcgcTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTc < 1:1787098/45‑1 (MQ=255) gcgcTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTc < 1:2092490/45‑1 (MQ=255) gcgcTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTc < 1:1909123/45‑1 (MQ=255) gcgcTTAAGCGTGTCAGCTAATGCCTGCTAATCAGGCTTGCCTTc < 1:2358240/45‑1 (MQ=255) | TGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAG > minE/1508645‑1508772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |