| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,513,411 | C→T | 58.8% | P156P (CCG→CCA) | ubiX ← | 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,513,411 | 0 | C | T | 58.8% | 19.4 / 44.3 | 63 | P156P (CCG→CCA) | ubiX | 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase |
| Reads supporting (aligned to +/- strand): ref base C (9/17); new base T (7/30); total (16/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
GGTTATCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAGGCATAATCACCGCACCGATTTCTGCCGCCTGAGTCATTA > minE/1513348‑1513478 | ggTTATCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCg < 1:352816/69‑1 (MQ=255)ggTTATCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCg < 1:603385/69‑1 (MQ=255)ggTTATCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCg < 1:2618119/69‑1 (MQ=255) aTCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCgatg > 1:2006216/1‑68 (MQ=255) aTCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCgatg > 1:420520/1‑68 (MQ=255) aTCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCgatg > 1:461509/1‑68 (MQ=255) aTCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCgatg > 1:3393792/1‑68 (MQ=255) aaGAACACGATTAACCGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAAc > 1:952333/1‑69 (MQ=255) aaCACGATTAACCGTCTGATTTATCACATCATCAAGGTATTGCGGGCGATGATAAAACGCCGGAACggg < 1:3108494/69‑1 (MQ=255) acacGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGa > 1:654965/1‑69 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:2763752/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:1037285/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:3244141/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:3206550/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:2600354/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:3194309/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:974188/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:1902506/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:1566944/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:849542/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAgg < 1:1414893/69‑1 (MQ=255) acGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACCCCGGAACGGGAgg < 1:181720/69‑1 (MQ=255) cGATTAACCGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAgg > 1:1219997/1‑68 (MQ=255) cGATTAACCGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAgg > 1:1735438/1‑68 (MQ=255) cGATTAACCGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAgg > 1:1713739/1‑68 (MQ=255) cGATTAACCGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAgg > 1:1693055/1‑68 (MQ=255) cGATTAACCGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAgg > 1:3420273/1‑68 (MQ=255) cGATTAACCGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAgg > 1:2703281/1‑68 (MQ=255) ccGTCTGATTTATCGCATCATCAAGTGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:30085/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:604963/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:2770672/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:311815/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:884421/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:3218808/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:3417339/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:368957/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:373736/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:437071/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:456770/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:250623/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:1098860/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:1183021/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:1483392/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:1599718/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:1610405/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:1713406/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:2423944/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:2473491/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:3094170/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:2613813/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:2617818/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:2654700/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:2669330/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:293230/68‑1 (MQ=255) ccGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:3031257/68‑1 (MQ=255) cGTCTGATTTATCGCATCATCAAGGGATTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:3263145/67‑1 (MQ=255) gTCTGATTTATCGCATCATCAAGGGAGCGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:2917120/66‑1 (MQ=255) tGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAGGCATAATcaccgca > 1:1353730/1‑69 (MQ=255) tGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAGGCATAATcaccgc > 1:2673106/1‑68 (MQ=255) tGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAGGCATAATcaccgc > 1:2965833/1‑68 (MQ=255) atcaAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAGGCATAATCACCGCACCGATTTCTgccgc > 1:2525897/1‑69 (MQ=255) aTTGTGGGCGATGATAAAACGCCGGAACGGGAGGCATAATc < 1:65921/41‑1 (MQ=255) gCGGGCGATGATAAAACGCCGGAACGGGAGGCATAATCACCGCACCGATTTCTGCCGCCTGAGTCATTa < 1:1714219/69‑1 (MQ=255) | GGTTATCGCAAACTGGTCAAGAACACGATTAACCGTCTGATTTATCACATCATCAAGGGATTGCGGGCGATGATAAAACGCCGGAACGGGAGGCATAATCACCGCACCGATTTCTGCCGCCTGAGTCATTA > minE/1513348‑1513478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |