| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,513,603 | A→C | 43.9% | L92L (CTT→CTG) | ubiX ← | 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,513,603 | 0 | A | C | 43.9% | 12.0 / 88.8 | 82 | L92L (CTT→CTG) | ubiX | 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (22/24); new base C (25/11); total (47/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01 | |||||||||||
ACATCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCT > minE/1513550‑1513668 | acaTCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAgg > 1:1061054/1‑57 (MQ=255)acaTCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTttgattg > 1:1179485/1‑67 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAgg > 1:2073008/1‑68 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAgg > 1:1516332/1‑68 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAgg > 1:378061/1‑68 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAgg > 1:2592449/1‑68 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAgg > 1:700312/1‑68 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAgg > 1:767710/1‑68 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAgg > 1:2011901/1‑68 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAgg > 1:1086754/1‑68 (MQ=255) tGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAg > 1:324555/1‑67 (MQ=255) ccATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGc < 1:1421418/69‑1 (MQ=255) ccATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGc < 1:2991838/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:1627560/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2059725/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:203301/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:3133391/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2712400/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:359905/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:373813/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:391275/69‑1 (MQ=255) aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2272120/69‑1 (MQ=255) tCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2131682/68‑1 (MQ=255) tCAGTATAGCTATGGACAATGCCGGACAGGGTTATGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2920938/68‑1 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCt > 1:2026013/1‑68 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCt > 1:3279795/1‑68 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCt > 1:172669/1‑68 (MQ=255) aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCt > 1:2280/1‑68 (MQ=255) taGCTGTGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1649911/1‑68 (MQ=255) taGCTATGGACAATGCCGTACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1090709/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTttgattga > 1:944478/1‑35 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1926234/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1985818/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:2051487/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:3254714/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:2634726/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1446463/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:231500/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:221358/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:2249111/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1059742/1‑68 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGa > 1:1614697/1‑67 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGa > 1:1502128/1‑67 (MQ=255) taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGa > 1:1288362/1‑67 (MQ=255) gatATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:2304641/3‑69 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTgg > 1:2350745/1‑64 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:2191377/68‑1 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:2633444/68‑1 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:3076012/68‑1 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:1012754/68‑1 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:1726828/68‑1 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:1950340/68‑1 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:2378911/1‑69 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:3425001/1‑69 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:1441631/1‑69 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:1658271/1‑69 (MQ=255) gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACAATCCCCAGCGTCTGGAAAg < 1:1040317/68‑1 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg > 1:2088917/1‑62 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg > 1:2092918/1‑62 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:1355533/1‑66 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:1105780/1‑66 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:3045857/1‑66 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:1785766/1‑66 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:2164679/1‑66 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:542232/1‑66 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:2595631/1‑66 (MQ=255) gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:333244/1‑66 (MQ=255) aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:1349378/69‑1 (MQ=255) aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:404864/69‑1 (MQ=255) aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:2577630/69‑1 (MQ=255) aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:3299660/69‑1 (MQ=255) aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:2775353/69‑1 (MQ=255) gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCTTCTGGAAAGAACCGGAAGAGAt < 1:1129244/69‑1 (MQ=255) gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:2488692/69‑1 (MQ=255) gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:1337440/69‑1 (MQ=255) gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:1506435/69‑1 (MQ=255) gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:3137907/69‑1 (MQ=255) gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:3038980/69‑1 (MQ=255) gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:1993897/69‑1 (MQ=255) ggAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCt < 1:1430321/69‑1 (MQ=255) ggAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCt < 1:423617/69‑1 (MQ=255) ggAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCt < 1:682057/69‑1 (MQ=255) | ACATCTGCCGCACGGGTCAGTAAGCCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCT > minE/1513550‑1513668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |