| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,514,054 | G→A | 34.5% | L479L (CTC→CTT) | purF ← | amidophosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,514,054 | 0 | G | A | 34.5% | 100.9 / 74.2 | 130 | L479L (CTC→CTT) | purF | amidophosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (20/65); new base A (28/17); total (48/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 141 total aligned reads displayed.
TTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCAAA > minE/1513990‑1514122 | tttCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGa > 1:3170/1‑69 (MQ=255)tttCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGa > 1:3197977/1‑69 (MQ=255)tttCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGa > 1:1395409/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCg > 1:1830335/1‑68 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCg > 1:3207894/1‑68 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:3319767/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:617607/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:54423/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:1925691/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:2057438/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:3340272/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:2184654/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:1028691/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:2269804/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:2363571/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:2987142/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:2907528/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:1189688/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:1462073/1‑69 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:900879/68‑1 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:2321138/68‑1 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:1250265/68‑1 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:540564/68‑1 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:268146/68‑1 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:1505657/68‑1 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:1577571/68‑1 (MQ=255) gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:839451/68‑1 (MQ=255) gaTTTTCCACTTCATTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa > 1:3087875/1‑69 (MQ=255) ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc < 1:1982426/68‑1 (MQ=255) ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc < 1:1519833/68‑1 (MQ=255) ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc < 1:1188625/68‑1 (MQ=255) ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc < 1:1013989/68‑1 (MQ=255) ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc < 1:2090590/68‑1 (MQ=255) ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc < 1:1336135/68‑1 (MQ=255) ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc < 1:573117/68‑1 (MQ=255) aCGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg < 1:1574104/49‑1 (MQ=255) aCGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATc > 1:1066177/1‑69 (MQ=255) aCTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt < 1:916036/68‑1 (MQ=255) aCTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt < 1:2150684/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGTTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1886549/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:2482994/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1098826/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1146916/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1327528/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:2384956/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:2580171/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:293505/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:3161153/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:333359/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa > 1:125876/1‑68 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:2093022/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:3353418/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1942951/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1933605/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1885526/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:850862/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1424343/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:171894/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:1576849/68‑1 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAg > 1:494987/1‑69 (MQ=255) ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAg > 1:1439583/1‑69 (MQ=255) tGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa < 1:2387347/67‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:2859624/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:944671/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:929222/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:1571749/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:724819/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:1832379/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:2254192/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:2479629/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:2904902/68‑1 (MQ=255) cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:2838237/68‑1 (MQ=255) atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGccat > 1:652257/1‑66 (MQ=255) atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCg > 1:473672/1‑67 (MQ=255) atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGt > 1:2073931/1‑68 (MQ=255) atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGt > 1:835625/1‑68 (MQ=255) atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGt > 1:1402661/1‑68 (MQ=255) cgtaaagtaTCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg < 1:3275568/57‑1 (MQ=255) taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg > 1:2643284/1‑68 (MQ=255) taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg > 1:1948669/1‑68 (MQ=255) taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg > 1:297911/1‑68 (MQ=255) taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg > 1:1664484/1‑68 (MQ=255) taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg > 1:2726290/1‑68 (MQ=255) aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt < 1:2239454/44‑1 (MQ=255) aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt < 1:2820401/44‑1 (MQ=255) aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt < 1:44434/44‑1 (MQ=255) aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt < 1:1830472/44‑1 (MQ=255) aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAg < 1:618897/69‑1 (MQ=255) aacgtaTCGAGGAAATCGAGGTAGACCTGATCAACATCTTTGGt < 1:1359174/44‑1 (MQ=255) acgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt < 1:1698700/43‑1 (MQ=255) cGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaa > 1:2387473/1‑69 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa > 1:2842164/1‑42 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa > 1:2567167/1‑42 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa > 1:3150878/1‑42 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa > 1:369066/1‑69 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa > 1:2089473/1‑69 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa > 1:2808918/1‑69 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa > 1:3242281/1‑69 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa > 1:3087165/1‑69 (MQ=255) gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa > 1:1126486/1‑69 (MQ=255) | TTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCAAA > minE/1513990‑1514122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |