| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,514,504 | G→A | 24.5% | R329R (CGC→CGT) | purF ← | amidophosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,514,504 | 0 | G | A | 24.5% | 107.3 / 27.8 | 85 | R329R (CGC→CGT) | purF | amidophosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (43/21); new base A (13/8); total (56/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
CAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATT > minE/1514438‑1514555 | cAGTTTGCGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:439275/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAGGGTGCGGCCAACATAGCg > 1:795112/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:1005773/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:3050994/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:2981604/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:3405561/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:37423/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:2058423/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:473939/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:513813/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:723794/1‑69 (MQ=255)cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:849744/1‑69 (MQ=255) gTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCgg < 1:3289844/68‑1 (MQ=255) tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:1085613/1‑68 (MQ=255) tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:2910099/1‑68 (MQ=255) tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:747487/1‑68 (MQ=255) tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:2696448/1‑68 (MQ=255) tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:2368440/1‑68 (MQ=255) tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:1153366/1‑68 (MQ=255) cGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGc < 1:3084279/69‑1 (MQ=255) cGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGc < 1:215394/69‑1 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAg > 1:2363968/1‑68 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2299822/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2152108/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2383278/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:1122243/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:571533/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2554709/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:1873110/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:703878/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:1783858/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:258230/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2610367/1‑69 (MQ=255) cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAAAATAACGGTTTTTAACGAAGc > 1:1194744/1‑69 (MQ=255) ggACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGc < 1:2445857/68‑1 (MQ=255) gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:39067/68‑1 (MQ=255) gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:2018453/68‑1 (MQ=255) gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:1158219/68‑1 (MQ=255) gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:3113635/68‑1 (MQ=255) gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:1568739/68‑1 (MQ=255) gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:758319/68‑1 (MQ=255) gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:1629795/68‑1 (MQ=255) gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:2879268/68‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAg < 1:1122308/67‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAg < 1:1138310/67‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAg < 1:862958/67‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAg < 1:1709569/67‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:1166213/69‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:1439484/69‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:3302288/69‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:1736031/69‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:2236930/69‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:607528/69‑1 (MQ=255) ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:2206109/69‑1 (MQ=255) tgCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:1076379/68‑1 (MQ=255) ggCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:1949757/69‑1 (MQ=255) ggCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:501968/69‑1 (MQ=255) ggCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:1958111/69‑1 (MQ=255) ggCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:2423907/69‑1 (MQ=255) aTGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATAc > 1:3243563/1‑67 (MQ=255) aTAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCt > 1:650472/1‑40 (MQ=255) aTAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCa > 1:3400126/1‑69 (MQ=255) aTAAAGGTCCCGCCAACATAGCGGTTTTTAACGAAGCCCTcgcg > 1:3423979/1‑44 (MQ=255) ggTGCGGCCAACATAGGGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:1920710/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:1789511/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:975374/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:1666037/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:697490/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:1261429/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:1241209/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:1097652/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:894585/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:1945089/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:2369864/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:3330652/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:3305222/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:3299/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:3135424/1‑67 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAt > 1:241865/1‑66 (MQ=255) ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAt > 1:1615783/1‑66 (MQ=255) ggTGCGGCCAACATAGAGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAAtt > 1:1770352/1‑67 (MQ=255) tAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTg > 1:2150437/1‑36 (MQ=255) tAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTg > 1:2964689/1‑36 (MQ=255) tAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTg > 1:2990184/1‑36 (MQ=255) aGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:2774382/44‑1 (MQ=255) | CAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATT > minE/1514438‑1514555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |