| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,517,661 | A→C | 42.9% | G178G (GGT→GGG) | folC ← | bifunctional folylpolyglutamate synthase and dihydrofolate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,517,661 | 0 | A | C | 42.9% | 10.9 / 74.2 | 70 | G178G (GGT→GGG) | folC | bifunctional folylpolyglutamate synthase and dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base A (10/30); new base C (27/3); total (37/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01 | |||||||||||
CAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAA > minE/1517597‑1517722 | cAATTGCCGGTTTTTCGCTGCGAAAGATACCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGCCcca < 1:1383689/69‑1 (MQ=255)cAATTGCCGGTTTTTCGCTGCGAAAGATACCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGCCcca < 1:3204737/69‑1 (MQ=255)cAATTGCCGGTTTTTCGCTGCGAAAGATACCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGCCcca < 1:739248/69‑1 (MQ=255) gTTTTTCGCTGCGGTAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:1387789/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:3001886/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:2080945/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:2220418/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:2447068/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:2883466/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:1618575/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:593902/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:1551327/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:679775/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:680834/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:877926/69‑1 (MQ=255) gTTTTTCGCTGCGGAAGATGCATGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCgg < 1:1899391/69‑1 (MQ=255) aaGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAgcgaa < 1:2740467/69‑3 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCATTCGGTATGATCCAGCGCaa < 1:1546370/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:2559700/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:936460/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:2724197/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:255258/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:2549084/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:2529804/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:752175/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:1981374/69‑1 (MQ=255) aGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCaa < 1:1484902/69‑1 (MQ=255) gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAAt > 1:2018014/1‑69 (MQ=255) gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAAt > 1:863808/1‑69 (MQ=255) gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAAt > 1:625475/1‑69 (MQ=255) gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAAt > 1:3215438/1‑69 (MQ=255) gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAAt > 1:1686984/1‑69 (MQ=255) gATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAAt > 1:1827224/1‑69 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGt > 1:2989444/1‑69 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTgg > 1:3023687/1‑68 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGt > 1:1594387/1‑69 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGt > 1:2596705/1‑69 (MQ=255) tGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGt > 1:980727/1‑69 (MQ=255) ttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATg < 1:261384/47‑1 (MQ=255) ttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTtac < 1:1415302/68‑1 (MQ=255) ttCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTtac < 1:1621928/68‑1 (MQ=255) tcGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTtactac < 1:1886334/68‑1 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCTCAATACTGGTTACTACCGCGACATc > 1:2511531/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATAc > 1:1941898/1‑49 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATAc > 1:2033952/1‑49 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACAt > 1:1939426/1‑67 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:853237/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:2214140/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:828071/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:1452718/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:1388487/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:2377907/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:1511991/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:1524887/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:660863/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:985904/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:3385844/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:471089/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:3424142/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:3338583/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:1594877/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:3142613/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:1926984/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:269594/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:2664435/1‑68 (MQ=255) aTACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:2589828/1‑68 (MQ=255) aTACTATCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATc > 1:2682472/1‑68 (MQ=255) tACTTTCGCGATCTGGCCCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCgg > 1:1305285/1‑69 (MQ=255) gATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACaa < 1:85657/69‑1 (MQ=255) gATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACaa < 1:171761/69‑1 (MQ=255) | CAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCGCGACCAATACTTTCGCGATCTGGACCCAGCCAGTCGGTATGATCCAGCGCAATACTGGTTACTACCGCGACATCGGCGTCGACAA > minE/1517597‑1517722 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |