| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,521,041 | C→A | 51.3% | S287S (TCG→TCT) | usg ← | predicted semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,521,041 | 0 | C | A | 51.3% | 0.1 / 97.3 | 78 | S287S (TCG→TCT) | usg | predicted semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (24/14); new base A (23/17); total (47/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACA > minE/1520983‑1521107 | cTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATc < 1:1019196/67‑1 (MQ=255)cTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATc < 1:2732666/67‑1 (MQ=255)cTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATc < 1:2324935/67‑1 (MQ=255)cTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATc < 1:188840/67‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGACGCATCa < 1:440425/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCa < 1:3324898/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCa < 1:2834605/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCa < 1:2367269/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCa < 1:2348780/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCa < 1:2318018/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCa < 1:1183177/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCa < 1:822343/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCa < 1:812593/54‑1 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTggg > 1:1449471/1‑69 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTggg > 1:507469/1‑69 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTggg > 1:2316729/1‑69 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTggg > 1:812344/1‑69 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTggg > 1:2964536/1‑69 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTggg > 1:2968388/1‑69 (MQ=255) ccGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTggg > 1:3306948/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:1608720/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:8543/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:2880008/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:2341537/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:231634/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:688983/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:190093/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:13738/1‑69 (MQ=255) aTTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGta > 1:3248429/1‑68 (MQ=255) acgcacgcAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:2501395/1‑66 (MQ=255) acgcacgcAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGtt > 1:2887839/1‑66 (MQ=255) acgcacgcAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTtc > 1:1840170/1‑67 (MQ=255) acgcacgcAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTtc > 1:1068948/1‑67 (MQ=255) acgcacgcAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTtc > 1:645991/1‑67 (MQ=255) acgcacgcAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTtc > 1:554177/1‑67 (MQ=255) acgcacgcAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTtc > 1:2127598/1‑67 (MQ=255) acgcacgcAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTtc > 1:2056321/1‑67 (MQ=255) acgcaGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCa < 1:586602/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:3345194/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:2937442/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:2942372/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:83760/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:2946196/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:673860/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:104477/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:2636568/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:1051645/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:1637491/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:1286154/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:1720444/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:1723841/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:1780092/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:2279450/69‑1 (MQ=255) caGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:2368990/69‑1 (MQ=255) aGCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGTCGGGAATTCGTTCTCTTCagag < 1:2821913/68‑1 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:748881/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:2434335/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:2431918/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:1241144/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:631463/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:624308/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:276878/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:2431650/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:1792673/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:400520/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:83741/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:1963662/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:2000350/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:881412/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:894319/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:3013989/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:2122933/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:2164771/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:959225/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:2222749/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGtc > 1:287370/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGt > 1:1893761/1‑45 (MQ=255) gCCAACGGAAAGATGCGGCGTACCAGAAGCATCTCCTACCTGAGt > 1:914394/1‑45 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCAGAATATCTTCGCCTTGAACa < 1:303658/69‑1 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAAc > 1:581192/1‑68 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa > 1:968523/1‑69 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa > 1:1355159/1‑69 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa < 1:1230570/69‑1 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa < 1:852804/69‑1 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa > 1:754695/1‑69 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa > 1:1651681/1‑69 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa < 1:1691770/69‑1 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa > 1:1951754/1‑69 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa < 1:3276610/69‑1 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa < 1:3140914/69‑1 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa < 1:3091120/69‑1 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa > 1:3074098/1‑69 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa < 1:2067073/69‑1 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa > 1:2209096/1‑69 (MQ=255) cccGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACa > 1:2637590/1‑69 (MQ=255) ccGAAGCATCACCTACCTGAGTTGTGAATTCGTTCTCTTCATAGAGCACAATATCTTCGCCTTGTACa < 1:3179286/68‑1 (MQ=255) | CTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACA > minE/1520983‑1521107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |