| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,525,931 | C→T | 84.8% | E309E (GAG→GAA) | fabB ← | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,525,931 | 0 | C | T | 84.8% | 61.0 / 3.2 | 39 | E309E (GAG→GAA) | fabB | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base T (13/20); total (17/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 | |||||||||||
CGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAAC > minE/1525864‑1525996 | cGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGct > 1:1436401/1‑69 (MQ=255)cGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGct > 1:3350954/1‑69 (MQ=255) ggCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCAc > 1:934928/1‑69 (MQ=255) gggCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTa < 1:1212570/69‑1 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCa > 1:2904024/1‑43 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:1034582/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:2768859/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:231724/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:2174945/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:1978799/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:188908/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:298593/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:3290757/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:11365/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:1087341/1‑68 (MQ=255) ctctTATCGCCGAACACTTCACGGATAGCTGCAAGTTAttt > 1:3249330/1‑41 (MQ=37) ctctTATCGCCGAACACTTCACGGATAGCAGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc > 1:1974557/1‑68 (MQ=255) gCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTggg > 1:98699/1‑65 (MQ=255) cacTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTa < 1:661929/69‑1 (MQ=255) acTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTaa > 1:3093953/1‑69 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAAGCGGAGTCGAAGTACGATGAGAGTTCa < 1:103747/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:338688/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:1121523/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:899351/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:879397/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:687456/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:621921/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:369628/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:356029/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:2413806/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:1449210/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:1512845/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:1561344/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:1580828/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:2905933/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:1913049/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:288190/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:2640326/58‑1 (MQ=255) cGGATAGCTGCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACAATGAGAGTTCa < 1:536045/58‑1 (MQ=255) cGGATAGCTCCCAGTTCTTTCACGTCGCCAACCGGAGTCGAAGTACCATGAGAGTTCa < 1:629220/58‑1 (MQ=255) aGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAAc > 1:2390754/1‑68 (MQ=255) | CGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACCGTGGGAGTTCAGGTAATCGATTGGGGTATCAAC > minE/1525864‑1525996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |