| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,527,117 | G→A | 39.9% | G34G (GGG→GGA) | trmC → | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,527,117 | 0 | G | A | 39.9% | 29.1 / 42.3 | 65 | G34G (GGG→GGA) | trmC | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base G (16/23); new base A (16/10); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
TGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCC > minE/1527057‑1527182 | tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:1750317/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:1889511/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:3026482/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:2613969/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:2348161/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:1796022/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:1787324/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:1776427/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:1762118/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:569115/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:1528659/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:756651/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:797692/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:26210/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTCGAaga > 1:1011357/1‑69 (MQ=255)tGCTGAGGGTACACCTGTTTCCAGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAaga > 1:2620146/1‑69 (MQ=255) gCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTTGAaga < 1:2321035/68‑1 (MQ=255) gCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAaga < 1:2315022/68‑1 (MQ=255) acacCTGTTTCCTGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGCAGAGACGCGatat > 1:1626070/1‑69 (MQ=255) acacCTGTTTCCTGAGATTTT‑ACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATg > 1:2230094/1‑69 (MQ=255) acacCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTAt > 1:3092838/1‑69 (MQ=255) acacCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTAt > 1:2730135/1‑69 (MQ=255) acacCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTAt > 1:775981/1‑69 (MQ=255) tCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCt > 1:3232994/1‑68 (MQ=255) tCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCt > 1:3015066/1‑68 (MQ=255) tCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCt > 1:472597/1‑68 (MQ=255) tCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCt > 1:2704776/1‑68 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:2606933/68‑1 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:1143947/68‑1 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:2807854/68‑1 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:2130575/68‑1 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:298858/68‑1 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:2053813/68‑1 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:32455/68‑1 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:1637551/68‑1 (MQ=255) cGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:1210891/68‑1 (MQ=255) gagaTTTTGACGATGTCTATTTTTCCAACGATAACGGACTGGAAGAGACGCGTTATGTTTTTCTcgg < 1:2651552/67‑1 (MQ=255) gTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTag > 1:2168777/1‑67 (MQ=255) tCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg < 1:3058430/69‑1 (MQ=255) tCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg < 1:3346109/69‑1 (MQ=255) tCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg > 1:2558117/1‑69 (MQ=255) tCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg > 1:2070570/1‑69 (MQ=255) tCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg < 1:1809937/69‑1 (MQ=255) tCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg < 1:1820462/69‑1 (MQ=255) tATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTTTGTTTTTCTGGGAGGCAACCAATTAGAGGt > 1:645850/1‑68 (MQ=255) tATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCt > 1:1859498/1‑47 (MQ=255) tATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTa > 1:1375563/1‑69 (MQ=255) tATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTa > 1:2099880/1‑69 (MQ=255) aTAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGc < 1:906408/69‑1 (MQ=255) aTAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGc < 1:1275732/69‑1 (MQ=255) aTAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGc < 1:2756322/69‑1 (MQ=255) aTAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGc < 1:1586100/69‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACTCtt < 1:2630517/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:3184500/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:1894577/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:199770/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:593506/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:62409/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:634206/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:1422242/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:228163/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:2684762/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:949624/58‑1 (MQ=255) aCGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:974038/58‑1 (MQ=255) cGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCtt < 1:705950/57‑1 (MQ=255) gggCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATcc < 1:186705/68‑1 (MQ=255) gggCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATcc < 1:3293364/68‑1 (MQ=255) gggCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATcc < 1:2370637/68‑1 (MQ=255) gggCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATc < 1:2089901/67‑1 (MQ=255) | TGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCC > minE/1527057‑1527182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |