| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,527,855 | G→A | 100% | S280S (TCG→TCA) | trmC → | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,527,855 | 0 | G | A | 76.2% | 53.5 / 12.9 | 46 | S280S (TCG→TCA) | trmC | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base G (1/10); new base A (34/1); total (35/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACT > minE/1527792‑1527917 | tatgggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTgg < 1:2390052/65‑1 (MQ=255) gggCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCt < 1:138802/69‑1 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCt > 1:615609/1‑68 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:2606371/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:928641/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:450724/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:357443/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:3370903/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:3366634/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:3012588/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:2620802/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:254795/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:2503185/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:2457787/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:2169908/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:1253366/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:1585926/1‑69 (MQ=255) ggCAGCAACAAACAGGAAGCGGCAATTATTGGAGGCGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTa > 1:1868333/1‑69 (MQ=255) gcagcagcaAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTAt < 1:57909/69‑1 (MQ=255) aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcgc < 1:3078851/69‑1 (MQ=255) aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcgc < 1:547191/69‑1 (MQ=255) aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcgc < 1:546325/69‑1 (MQ=255) aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcgc < 1:2029145/69‑1 (MQ=255) gCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg < 1:2085384/69‑1 (MQ=255) gCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg < 1:902956/69‑1 (MQ=255) gCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg < 1:2788132/69‑1 (MQ=255) gCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg < 1:2797754/69‑1 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAg > 1:1304317/1‑42 (MQ=37) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTGTTGCGCgg > 1:3136566/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTAt > 1:1907035/1‑54 (MQ=38) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:1215840/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:1300823/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:2469039/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:1343247/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:1490643/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:3279625/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:3144857/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:3098534/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:2124634/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:307018/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:1594879/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:3009267/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:1708951/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:2602510/1‑61 (MQ=255) aGCGCGTTGTTGTCACTGGAGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg > 1:2439845/1‑61 (MQ=38) gttgtCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACt > 1:3278879/1‑69 (MQ=255) | CACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACT > minE/1527792‑1527917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |