Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,527,870 G→T 100% R285R (CGG→CGT)  trmC → fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,527,8700GT75.0% 29.1 / 8.3 24R285R (CGG→CGT) trmCfused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base T (18/0);  total (19/5)
Fisher's exact test for biased strand distribution p-value = 1.41e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCT  >  minE/1527805‑1527924
                                                                 |                                                      
aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcgc                                                     <  1:547191/69‑1 (MQ=255)
aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcgc                                                     <  1:546325/69‑1 (MQ=255)
aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcgc                                                     <  1:3078851/69‑1 (MQ=255)
aaaCGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGgcgc                                                     <  1:2029145/69‑1 (MQ=255)
                                 gCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg                    <  1:2788132/69‑1 (MQ=255)
                                 gCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg                    <  1:902956/69‑1 (MQ=255)
                                 gCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg                    <  1:2797754/69‑1 (MQ=255)
                                 gCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg                    <  1:2085384/69‑1 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAg                                            >  1:1304317/1‑42 (MQ=37)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTGTTGCGCgg                         >  1:3136566/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTAt                                >  1:1907035/1‑54 (MQ=38)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:1490643/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:1300823/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:1343247/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:3279625/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:3144857/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:3098534/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:307018/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:3009267/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:1594879/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:1215840/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:2602510/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:2469039/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:2124634/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGCGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:1708951/1‑61 (MQ=255)
                                    aGCGCGTTGTTGTCACTGGAGCTATTGCGTCGCGGCTGGCAGGTAACGCTTTATTGCGCgg                         >  1:2439845/1‑61 (MQ=38)
                                            gttgtCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACt         >  1:3278879/1‑69 (MQ=255)
                                                     ggCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCt  <  1:710429/67‑1 (MQ=255)
                                                                 |                                                      
AAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCT  >  minE/1527805‑1527924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑