| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,528,294 | T→C | 52.8% | Y427H (TAT→CAT) | trmC → | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,528,294 | 0 | T | C | 52.8% | 8.6 / 75.9 | 89 | Y427H (TAT→CAT) | trmC | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base T (32/10); new base C (18/29); total (50/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
GTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAG > minE/1528226‑1528360 | gtTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGAttt > 1:728205/1‑69 (MQ=255)gtTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGAttt > 1:3276679/1‑69 (MQ=255)gtTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGAttt > 1:825276/1‑69 (MQ=255) ggCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCATGGTTTGCAGATttatt < 1:1340622/69‑1 (MQ=255) ggCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatt < 1:1332541/69‑1 (MQ=255) ggCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatt < 1:610732/69‑1 (MQ=255) ggCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatt < 1:215414/69‑1 (MQ=255) ggCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatt < 1:831007/69‑1 (MQ=255) ggCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatt < 1:1097251/69‑1 (MQ=255) ggCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatt < 1:2646534/69‑1 (MQ=255) cagcagAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATc < 1:710814/69‑1 (MQ=255) agAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGt > 1:799877/1‑68 (MQ=255) agAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGt > 1:328500/1‑68 (MQ=255) agAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGt > 1:714566/1‑68 (MQ=255) ccGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAAttt < 1:845198/69‑1 (MQ=255) ccGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAAttt < 1:828729/69‑1 (MQ=255) gTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATttatt < 1:2323515/45‑1 (MQ=255) aTGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATcc > 1:2159017/1‑68 (MQ=255) aTGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATcc > 1:2250005/1‑68 (MQ=255) aTGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATcc > 1:1992707/1‑68 (MQ=255) gtgCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAAttt < 1:1579323/62‑1 (MQ=255) tgCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTa > 1:681536/1‑69 (MQ=255) tgCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTa > 1:2269083/1‑69 (MQ=255) tgCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTa > 1:526431/1‑69 (MQ=255) tgCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTa > 1:1078002/1‑69 (MQ=255) tgCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTa > 1:107992/1‑69 (MQ=255) tgCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTa > 1:1542790/1‑69 (MQ=255) cTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAg < 1:2743004/69‑1 (MQ=255) cTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAg < 1:1032090/69‑1 (MQ=255) cTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAg < 1:1118764/69‑1 (MQ=255) cTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAg < 1:2481142/69‑1 (MQ=255) cTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAg < 1:1445778/69‑1 (MQ=255) cTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAg < 1:259559/69‑1 (MQ=255) tcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1749269/68‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGTATGACGgttg < 1:49674/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:857359/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:2777374/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:3155835/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1016132/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:3183701/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:2721915/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:850927/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:829287/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:729583/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:3293616/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:3385901/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:351291/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:684119/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:668802/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1396556/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1364319/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1092342/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:2523679/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:170733/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1120028/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1561268/69‑1 (MQ=255) gcAACAGCAGGGTTTGCAGATTCATTATCAACATCACTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1840711/69‑1 (MQ=255) cAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1614018/68‑1 (MQ=255) gTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAtt > 1:2253574/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTgttggt > 1:3058894/1‑59 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGaa > 1:234649/1‑66 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:2134056/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:1752284/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:2687867/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:2153109/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:55845/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:14288/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:3055663/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:2805720/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:1041320/1‑69 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1334801/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1308462/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:829882/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:674221/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1020790/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:593478/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:500811/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:2050544/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:2595780/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:2726260/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:2535958/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:3016364/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:3146655/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:3269554/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1785630/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:449307/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1797580/1‑63 (MQ=255) cAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGc > 1:17011/1‑68 (MQ=255) aGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGc > 1:3426675/1‑67 (MQ=255) aGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGc > 1:68141/1‑67 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:1696796/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:3242739/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:3139708/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:1266579/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:45265/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:2988506/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:603113/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:526412/69‑1 (MQ=255) tttATTATCAATATCAGTTAAAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:676237/69‑1 (MQ=255) tttATTATCAATATCAGATACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:2503105/69‑1 (MQ=255) | GTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAG > minE/1528226‑1528360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |