| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,528,336 | T→G | 43.7% | C441G (TGT→GGT) | trmC → | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,528,336 | 0 | T | G | 43.7% | 19.6 / 122.7 | 110 | C441G (TGT→GGT) | trmC | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base T (38/24); new base G (18/30); total (56/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
GCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGA > minE/1528272‑1528396 | tcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1749269/68‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGTATGACGgttg < 1:49674/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1396556/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:460367/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:3155835/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:668802/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:351291/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:2523679/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:3293616/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1561268/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:684119/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:3183701/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1364319/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:729583/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:829287/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:2549034/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:2777374/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1120028/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1092342/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:850927/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:857359/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:2721915/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1016132/69‑1 (MQ=255)gcAACAGCAGGGTTTGCAGATTCATTATCAACATCACTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1840711/69‑1 (MQ=255) cAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:1614018/68‑1 (MQ=255) cAACAGCAGGGTTTGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:2944819/68‑1 (MQ=255) gTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAtt > 1:2253574/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGaa > 1:234649/1‑66 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:3055663/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:2687867/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:2805720/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:2153109/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:2134056/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:55845/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:1752284/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:14288/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAttt > 1:1041320/1‑69 (MQ=255) tttGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAAtt > 1:1125315/1‑68 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:3269554/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:449307/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:3146655/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:593478/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:3016364/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:671871/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:674221/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:829882/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1020790/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1785630/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:2595780/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:2535958/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1797580/1‑63 (MQ=255) tGCAGATTCATTATCAACATCAGTTACAGGATTTATCCCGTAAGGATGACGGTTGGTTGTTGa > 1:1334801/1‑63 (MQ=255) cAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGc > 1:17011/1‑68 (MQ=255) aGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGc > 1:3426675/1‑67 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:603113/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:1696796/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:3242739/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:3139708/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:771285/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:45265/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:1266579/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:2988506/69‑1 (MQ=255) tttATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:526412/69‑1 (MQ=255) tttATTATCAATATCAGTTAAAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:676237/69‑1 (MQ=255) tttATTATCAATATCAGATACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGgag < 1:2503105/69‑1 (MQ=255) tCAGTTACAGGATTTATCCCGTAAGGATGACGgttg < 1:3034704/36‑1 (MQ=255) tCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAgcaagcaa > 1:2689304/1‑68 (MQ=255) tCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAgcaagcaa > 1:642622/1‑68 (MQ=255) tCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAgcaagcaa > 1:1787081/1‑68 (MQ=255) tCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAgcaagcaa > 1:2105806/1‑68 (MQ=255) tCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAgcaagcaa > 1:3152465/1‑68 (MQ=255) tCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAgcaagcaa > 1:2403210/1‑68 (MQ=255) tCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAgcaagcaa > 1:844237/1‑68 (MQ=255) cAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCagcaagc > 1:3049438/1‑65 (MQ=255) cAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCagcaagc > 1:2950585/1‑65 (MQ=255) cAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCagcaagc > 1:1062262/1‑65 (MQ=255) aGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAaca > 1:2023199/1‑68 (MQ=255) aGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAaca > 1:2163134/1‑68 (MQ=255) aGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAaca > 1:2016327/1‑68 (MQ=255) aGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAaca > 1:144285/1‑68 (MQ=255) aGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAaca > 1:1717920/1‑68 (MQ=255) aGTTACAGAATTTATCCCCTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAaca > 1:873632/1‑68 (MQ=255) tACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAgcaagcaa > 1:1655704/1‑63 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAAGACACAg < 1:436845/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:2381773/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:2668562/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:2965787/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:2420597/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:792767/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:3099279/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:578386/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:2380941/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:1888685/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:459742/69‑1 (MQ=255) aCAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAg < 1:496199/69‑1 (MQ=255) gAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCg < 1:264973/68‑1 (MQ=255) tttATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGt > 1:328164/1‑69 (MQ=255) aGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAg > 1:1078504/1‑57 (MQ=255) aGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGa > 1:3385944/1‑69 (MQ=255) aGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGa > 1:1157562/1‑69 (MQ=255) | GCAACAGCAGGGTTTGCAGATTTATTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGA > minE/1528272‑1528396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |