Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,528,792 | T→C | 43.6% | L593L (TTG→CTG) | trmC → | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,528,792 | 0 | T | C | 43.6% | 20.2 / 74.3 | 94 | L593L (TTG→CTG) | trmC | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
Reads supporting (aligned to +/- strand): ref base T (41/12); new base C (19/22); total (60/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.50e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01 |
ATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTT > minE/1528730‑1528859 | atcatcTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCgg > 1:2813222/1‑69 (MQ=255) atcatcTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCgg > 1:2778441/1‑69 (MQ=255) atcatcTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCgg > 1:1137544/1‑69 (MQ=255) atcatcTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCgg > 1:2562230/1‑69 (MQ=255) atcatcTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCgg > 1:3285504/1‑69 (MQ=255) atcatcTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCgg > 1:1742285/1‑69 (MQ=255) aaTGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGaaa < 1:2922332/67‑1 (MQ=255) aaTGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGaaa < 1:2929940/67‑1 (MQ=255) aaTGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGaaa < 1:1795759/67‑1 (MQ=255) aaTGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGaaa < 1:917263/67‑1 (MQ=255) tGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATg > 1:2632481/1‑69 (MQ=255) gTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgc > 1:1998179/1‑69 (MQ=255) ttCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgc > 1:3033419/1‑68 (MQ=255) cccGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgcgcg > 1:21347/1‑69 (MQ=255) cccGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgcgcg > 1:1225308/1‑69 (MQ=255) ccagATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgcgcg > 1:2248414/4‑69 (MQ=255) ttATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGt > 1:1178874/1‑69 (MQ=255) ttATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGt > 1:3118411/1‑69 (MQ=255) ttATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGt > 1:259956/1‑69 (MQ=255) ttATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGt > 1:2926301/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:1823694/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:2370080/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:2435467/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:409734/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:1023028/1‑69 (MQ=255) tGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGa > 1:2926669/1‑41 (MQ=255) tGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGa > 1:261086/1‑41 (MQ=255) tGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGa > 1:2396239/1‑41 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACg > 1:2617552/2‑68 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:253670/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:1002584/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2938930/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2404594/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2387709/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2669450/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:1030685/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2881515/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:3074853/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2109277/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:60663/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:3185257/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:142136/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:925375/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:1910005/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:33363/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGAGCGCCGGt > 1:1791879/2‑60 (MQ=255) tacacTCGTCGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACg > 1:2768968/2‑68 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGa > 1:1925047/1‑68 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGa > 1:1450029/1‑68 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGAt > 1:133425/1‑69 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGAt > 1:1190555/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATg > 1:2345800/1‑35 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATctct > 1:28483/1‑68 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:388413/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:3273257/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:452346/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:544083/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:574527/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:2888256/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:587700/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:101151/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:2453372/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:1803519/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:1872300/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:2038848/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:1563473/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:2457217/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:1470255/69‑1 (MQ=255) gTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:2820441/68‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1517733/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:154134/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:743360/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2822454/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:826959/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:161139/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:881877/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1654586/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1660701/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:177720/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:369607/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1980522/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2748182/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2670233/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:255300/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2469330/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1983571/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:3201118/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:3234009/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:362710/69‑1 (MQ=255) aaTATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2125768/68‑1 (MQ=255) aaTATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2280702/68‑1 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTg > 1:849960/1‑68 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTg > 1:2729682/1‑68 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTg > 1:1358345/1‑68 (MQ=255) cgtcgtTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCgg > 1:849133/1‑69 (MQ=255) gtTGGCGGAGCAGAAAGATGAGGCGGTATGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCttt > 1:2722848/1‑69 (MQ=255) gtTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCttt > 1:999579/1‑69 (MQ=255) | ATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTT > minE/1528730‑1528859 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |