| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,528,812 | G→A | 45.0% | E599E (GAG→GAA) | trmC → | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,528,812 | 0 | G | A | 45.0% | 19.5 / 72.3 | 91 | E599E (GAG→GAA) | trmC | fused 5‑methylaminomethyl‑2‑thiouridine forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base G (33/17); new base A (19/22); total (52/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01 | |||||||||||
GTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGT > minE/1528753‑1528878 | gTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgc > 1:1998179/1‑69 (MQ=255) ttCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgc > 1:3033419/1‑68 (MQ=255) cccGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgcgcg > 1:21347/1‑69 (MQ=255) cccGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgcgcg > 1:1225308/1‑69 (MQ=255) ccagATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAgcgcg > 1:2248414/4‑69 (MQ=255) ttATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGt > 1:1178874/1‑69 (MQ=255) ttATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGt > 1:3118411/1‑69 (MQ=255) ttATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGt > 1:259956/1‑69 (MQ=255) ttATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGt > 1:2926301/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:2435467/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:1823694/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:2370080/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:1023028/1‑69 (MQ=255) tATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGtt > 1:409734/1‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACg > 1:2617552/2‑68 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:253670/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2938930/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:1910005/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2881515/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2109277/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:3074853/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2669450/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2387709/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:2404594/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:1002584/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:3185257/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:1030685/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:33363/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:925375/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:60663/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGa > 1:142136/2‑69 (MQ=255) tacacTCGTGGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGAGCGCCGGt > 1:1791879/2‑60 (MQ=255) tacacTCGTCGAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACg > 1:2768968/2‑68 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGa > 1:1450029/1‑68 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGa > 1:1925047/1‑68 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGAt > 1:133425/1‑69 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGAt > 1:1190555/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATctct > 1:28483/1‑68 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:452346/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:544083/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:574527/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:388413/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:587700/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:2888256/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:3273257/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:101151/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:2457217/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:1872300/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:1803519/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:1563473/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt > 1:2038848/1‑69 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:1470255/69‑1 (MQ=255) cGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:2453372/69‑1 (MQ=255) gTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:2820441/68‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1654586/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:743360/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1660701/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:3234009/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:3201118/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:826959/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:362710/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:177720/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:369607/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:161139/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:154134/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:881877/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1980522/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1983571/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2822454/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2748182/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:1517733/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2670233/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2469330/69‑1 (MQ=255) gAATATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:255300/69‑1 (MQ=255) aaTATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2125768/68‑1 (MQ=255) aaTATGCGTCGCTGGCGGAGAAGAAAGATGAAGCGGTAAGCGCGCCGGTTTATGACGATCTCTTTATg < 1:2280702/68‑1 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTg > 1:1358345/1‑68 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTg > 1:849960/1‑68 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTg > 1:2729682/1‑68 (MQ=255) cgtcgtTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCgg > 1:849133/1‑69 (MQ=255) gtTGGCGGAGCAGAAAGATGAGGCGGTATGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCttt > 1:2722848/1‑69 (MQ=255) gtTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCttt > 1:999579/1‑69 (MQ=255) cGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCtt < 1:1667721/49‑1 (MQ=255) aaaGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTGTTTATGTTTGCGGCTTTAGGTTCTCGCGGt < 1:3418086/69‑1 (MQ=255) aaaGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGt < 1:1353504/69‑1 (MQ=255) aaaGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGt < 1:636341/69‑1 (MQ=255) gAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTgtgt < 1:3103197/69‑1 (MQ=255) gAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTgtgt < 1:1804118/69‑1 (MQ=255) gAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTgtgt < 1:1916846/69‑1 (MQ=255) gAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTgtgt < 1:1277295/69‑1 (MQ=255) gAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTgtgt < 1:2351357/69‑1 (MQ=255) | GTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGT > minE/1528753‑1528878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |