Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,529,221 A→C 36.5% A67A (GCT→GCG)  yfcL ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,529,2210AC36.5% 29.1 / 42.7 52A67A (GCT→GCG) yfcLhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (14/19);  new base C (12/7);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTG  >  minE/1529154‑1529280
                                                                   |                                                           
aCGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAg                                                            >  1:735087/1‑69 (MQ=255)
aCGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAg                                                            >  1:2892735/1‑69 (MQ=255)
   ccTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCAc                                                         <  1:2449057/69‑1 (MQ=255)
    ctgctgAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCAcc                                                        <  1:390189/69‑1 (MQ=255)
            aCAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCtt                                                >  1:2991741/1‑69 (MQ=255)
            aCAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCtt                                                >  1:2307704/1‑69 (MQ=255)
            aCAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCtt                                                >  1:1816981/1‑69 (MQ=255)
            aCAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCtt                                                >  1:1724266/1‑69 (MQ=255)
            aCAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACAAAt                                                     >  1:2147162/1‑64 (MQ=255)
            aCAGGTGTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCtt                                                >  1:1128/1‑69 (MQ=255)
                  tttCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCa                                          >  1:2414813/1‑69 (MQ=255)
                      ccACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGAc                                       <  1:1895979/68‑1 (MQ=255)
                      ccACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGAc                                       <  1:873819/68‑1 (MQ=255)
                      ccACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGAc                                       <  1:1685874/68‑1 (MQ=255)
                      ccACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGAc                                       <  1:1054599/68‑1 (MQ=255)
                          atatCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTg                                   >  1:693037/1‑68 (MQ=255)
                          atatCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTg                                   >  1:375978/1‑68 (MQ=255)
                          atatCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTg                                   >  1:243717/1‑68 (MQ=255)
                           tatCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCAcc                                                        <  1:3323239/46‑1 (MQ=255)
                           tatCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCAcc                                                        <  1:966844/46‑1 (MQ=255)
                           tatCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCAcc                                                        <  1:2153958/46‑1 (MQ=255)
                           tatCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCAcc                                                        <  1:122858/46‑1 (MQ=255)
                           tatCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCAcc                                                        <  1:9073/46‑1 (MQ=255)
                           tatCGGTCACCAGCCCCTGGTCACGCGGCGACAATTCACCAGCAcc                                                        <  1:1476116/46‑1 (MQ=255)
                           tatCGGTCACCAGCACCTGGTCACGCGGCGACAATTCACCAGCAcc                                                        <  1:3359985/46‑1 (MQ=255)
                                      aGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTa                      >  1:1717842/1‑69 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATgg                                                   >  1:3022572/1‑39 (MQ=37)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:1619735/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:2831688/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:2197430/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:2118921/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:3301481/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:20817/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:1744965/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:2040647/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:1987156/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:1898416/1‑68 (MQ=255)
                                       gcgcCTGGTCACGCGGTGACAATTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTa                      >  1:1141408/1‑68 (MQ=255)
                                        cgcCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATg                    <  1:2778017/69‑1 (MQ=255)
                                        cgcCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATg                    <  1:3346363/69‑1 (MQ=255)
                                         gcCTGGTCACGCGGCGACAATTCACCAGCACCATTGGCTTTTTCCAGAGTCTGGCTAACGGTCGTATg                    <  1:618649/68‑1 (MQ=255)
                                            tGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCAcc                <  1:534212/69‑1 (MQ=255)
                                             ggTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCAcc                <  1:2724270/68‑1 (MQ=255)
                                                cACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGc              >  1:2519614/1‑67 (MQ=255)
                                                      gcgACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCgg      <  1:874159/69‑1 (MQ=255)
                                                           aaTTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTATGTACCGCCTGAGCGGAGTg  <  1:624053/68‑1 (MQ=255)
                                                           aaTTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTATGTACCGCCTGAGCGGAGTg  <  1:483136/68‑1 (MQ=255)
                                                           aaTTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTATGTACCGCCTGAGCGGAGTg  <  1:435577/68‑1 (MQ=255)
                                                           aaTTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTATGTACCGCCTGAGCGGAGTg  <  1:821134/68‑1 (MQ=255)
                                                           aaTTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTATGTACCGCCTGAGCGGAGTg  <  1:3431122/68‑1 (MQ=255)
                                                           aaTTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTATGTACCGCCTGAGCGGAGTg  <  1:1179411/68‑1 (MQ=255)
                                                           aaTTCACCCGCGCCAATGGCTTTTTCCAGACTCTGGCTAACGGTTGTATGTACCGCCTGAGCGGAGTg  <  1:977265/68‑1 (MQ=255)
                                                                   |                                                           
ACGCCTGCTGAAACAGGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTG  >  minE/1529154‑1529280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑