| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,530,201 | G→A | 100% | G204G (GGC→GGT) | yfcA ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,530,201 | 0 | G | A | 97.5% | 79.3 / ‑5.1 | 37 | G204G (GGC→GGT) | yfcA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (18/17); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGC > minE/1530152‑1530263 | ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCCCCgaga > 1:2883605/1‑56 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:995878/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:1045303/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:883012/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:697596/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:39789/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:348363/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:3342856/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:3289835/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:3226122/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:3055395/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:2884694/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:2638091/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:1162714/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:1926696/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:1905094/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:1896842/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:2500953/1‑67 (MQ=255)ggAACTGTCCTACCAACATCACAAATCCTGTCGCCCAAATCACTTTGCCACCGAGAATAAAcagcagca > 1:1732022/1‑67 (MQ=255) ccAAATCACTTTGCGACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:2648232/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCTGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:3190963/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:1736804/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:1410507/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:823294/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:763111/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:513196/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:507323/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:483483/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:1644227/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:1784644/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:3057616/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:1904919/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:1911239/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:2863955/68‑1 (MQ=255) ccAAATCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:2543129/68‑1 (MQ=255) aaTCACTTTGCCACCGAGAATAAACAGCAGCAAACCGCCAATGTTTGACGTTGCGTTAAGTAAtt < 1:1636599/65‑1 (MQ=255) tttGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGc < 1:34928/69‑1 (MQ=255) | GGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGC > minE/1530152‑1530263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |