| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,532,234 | C→T | 100% | P164P (CCG→CCA) | aroC ← | chorismate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,532,234 | 0 | C | T | 91.1% | 38.9 / ‑3.2 | 22 | P164P (CCG→CCA) | aroC | chorismate synthase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (0/20); total (1/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTT > minE/1532206‑1532300 | cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:2528438/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:461837/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:384659/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:3433037/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:3195152/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:2996289/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:2994602/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:2938176/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:277767/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:2646980/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:1098693/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:2307573/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:1796947/69‑1 (MQ=255)cGACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:1785016/69‑1 (MQ=255) gACCTGAGACCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCaa < 1:2470535/68‑1 (MQ=255) aCCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCAATGCCAAAt < 1:1585249/68‑1 (MQ=255) aCCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCAATGCCAAAt < 1:1511539/68‑1 (MQ=255) aCCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCAATGCCAAAt < 1:3226501/68‑1 (MQ=255) aCCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCAATGCCAAAt < 1:1375292/68‑1 (MQ=255) aCCAGTCTTTGATTTCCAGTGGAATGTCGCCCATCTGGGTCAGGCAACCGCGAATCTCAATGCCAAAt < 1:1358441/68‑1 (MQ=255) tGATATCCAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCg > 1:1286996/1‑65 (MQ=255) gCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATAtttt < 1:2305508/68‑1 (MQ=255) | CGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTCGCCCATCTGGGTCAGGCAGCCACGGATTTCAATACCAAATTTCTCGGCGAGATATTTT > minE/1532206‑1532300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |