| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 122,440 | C→T | 24.0% | A206A (GCC→GCT) | cueO → | multicopper oxidase (laccase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 122,440 | 0 | C | T | 24.0% | 33.4 / 5.7 | 29 | A206A (GCC→GCT) | cueO | multicopper oxidase (laccase) |
| Reads supporting (aligned to +/- strand): ref base C (9/13); new base T (5/2); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCAACGGTGCAATCTACCCGCA > minE/122375‑122490 | gTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTg < 1:413993/69‑1 (MQ=255)gTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTg < 1:1441017/69‑1 (MQ=255) tAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTg > 1:3349423/1‑61 (MQ=255) tAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTg > 1:2426090/1‑61 (MQ=255) tAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTg < 1:1869157/61‑1 (MQ=255) tAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTg < 1:2236370/61‑1 (MQ=255) tAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTg < 1:213195/61‑1 (MQ=255) aGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTg < 1:883869/69‑1 (MQ=255) aGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTg < 1:353830/69‑1 (MQ=255) aGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTg < 1:2779892/69‑1 (MQ=255) aTTTAACGCTGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCTGTGGGTTGGTTTGGCGa > 1:1948117/1‑69 (MQ=255) aTTTAACGCTGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCTGTGGGTTGGTTTGGCGa > 1:792481/1‑69 (MQ=255) aTTTAACGCTGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCTGTGGGTTGGTTTGGCGa > 1:124062/1‑69 (MQ=255) aTTTAACGCTGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCTGTGGGTTGGTTTGGCGa > 1:2934983/1‑69 (MQ=255) aTTTAACGCTGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCTGTGGGTTGGTTTGGCGa > 1:2313563/1‑69 (MQ=255) ttAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATa > 1:1584523/1‑69 (MQ=255) ttAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATa > 1:2969962/1‑69 (MQ=255) ttgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCACCGGt > 1:2719759/1‑68 (MQ=255) ttgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCAACGGt > 1:1576074/1‑68 (MQ=255) ttgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCAACGGt > 1:2164394/1‑68 (MQ=255) ttgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCAACGGt > 1:678607/1‑68 (MQ=255) ttgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCAACGGt > 1:842583/1‑68 (MQ=255) tgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTgg < 1:160962/40‑1 (MQ=255) tgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTgg < 1:2213750/40‑1 (MQ=255) tgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTgg < 1:3104307/40‑1 (MQ=255) tgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTgg < 1:2125439/40‑1 (MQ=255) tgattATCAACTGGATGTGATGACCGCCGCCGTGGGCTgg < 1:1019753/40‑1 (MQ=255) ggATGTGATGACCGCCGCTGTGGGTTGGTTTGGCGATACGTTGCTGACCAACGGTGCAATCTACCCGCa < 1:1873106/69‑1 (MQ=255) gATGTGATGACCGCCGCTGTGGGTTGGTTTGGCGATACGTTGCTGACCAACGGTGCAATCTACCCGCa < 1:1551183/68‑1 (MQ=255) | GTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGATGTGATGACCGCCGCCGTGGGCTGGTTTGGCGATACGTTGCTGACCAACGGTGCAATCTACCCGCA > minE/122375‑122490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |