| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,533,152 | G→A | 36.5% | L181L (CTG→TTG) | prmB ← | N5‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,533,152 | 0 | G | A | 36.5% | 127.4 / 112.5 | 183 | L181L (CTG→TTG) | prmB | N5‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (63/53); new base A (46/21); total (109/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 184 total aligned reads displayed.
CGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATCCACCGCGTCGAC > minE/1533089‑1533218 | gTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg < 1:2593422/69‑1 (MQ=255) gTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg < 1:568040/69‑1 (MQ=255) gTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg < 1:1501175/69‑1 (MQ=255) gTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg < 1:2907699/69‑1 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:2845556/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:1951751/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:1733787/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:2660410/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:1628321/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:2757169/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:1367318/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:1330850/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:40769/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:647969/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtgt > 1:1319675/1‑69 (MQ=255) tACTGCACTTTCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCgtg > 1:724065/1‑68 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg > 1:1008711/1‑66 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg > 1:200870/1‑66 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg > 1:3059817/1‑66 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg > 1:2264980/1‑66 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg > 1:2314940/1‑66 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg > 1:1779703/1‑66 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg > 1:662171/1‑66 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgtg > 1:685225/1‑66 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttc > 1:165129/1‑69 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttc > 1:1922322/1‑69 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttc > 1:2480535/1‑69 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttc > 1:1716220/1‑69 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttc > 1:1511857/1‑69 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttc > 1:3016816/1‑69 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGtt > 1:1815499/1‑68 (MQ=255) cTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGtt > 1:3286386/1‑68 (MQ=255) cTGCACTTTCGG‑AAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCgt > 1:553202/1‑64 (MQ=255) cACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttcttc > 1:2805708/1‑69 (MQ=255) cACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttcttc > 1:3245693/1‑69 (MQ=255) cTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttcttc < 1:954796/67‑1 (MQ=255) cTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttcttc < 1:2467392/67‑1 (MQ=255) cTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttcttc < 1:3316758/67‑1 (MQ=255) cTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttcttc < 1:1277913/67‑1 (MQ=255) cTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttcttc < 1:1139468/67‑1 (MQ=255) cTTTCCGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGttcttc < 1:2805414/67‑1 (MQ=255) ttCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtg > 1:1909891/1‑69 (MQ=255) ttCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtg > 1:1416907/1‑69 (MQ=255) ttCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtg > 1:2689944/1‑69 (MQ=255) ttCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtg > 1:297130/1‑69 (MQ=255) ttCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtg > 1:2853453/1‑69 (MQ=255) ttCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAt > 1:2696370/1‑68 (MQ=255) tCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGAtgt > 1:2285258/1‑69 (MQ=255) tCGGCAAGTCACGGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGAtgt > 1:1202990/1‑69 (MQ=255) cgcgGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCa > 1:2172741/1‑68 (MQ=255) acgGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAg > 1:1102662/2‑68 (MQ=255) acgGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGc > 1:2786847/2‑69 (MQ=255) acgGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGc > 1:767846/2‑69 (MQ=255) acgGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGc > 1:252536/2‑69 (MQ=255) acgGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGc > 1:2499757/2‑69 (MQ=255) acgGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGc > 1:598247/2‑69 (MQ=255) acgGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGc > 1:2339952/2‑69 (MQ=255) acgGAACAAGTCGGAACGAATCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGc > 1:1208666/2‑69 (MQ=255) ggAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCa < 1:3169433/68‑1 (MQ=255) ggAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCa < 1:479611/68‑1 (MQ=255) ggAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCa < 1:3333168/68‑1 (MQ=255) ggAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCa < 1:339804/68‑1 (MQ=255) ggAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCa < 1:1864424/68‑1 (MQ=255) ggAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCa < 1:189935/68‑1 (MQ=255) aCAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAGcc > 1:53936/1‑68 (MQ=255) aCAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAAcc > 1:195910/1‑68 (MQ=255) aTCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAg < 1:724333/69‑1 (MQ=255) aTCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAg < 1:379388/69‑1 (MQ=255) aTCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAg < 1:2037400/69‑1 (MQ=255) tCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAg < 1:2350741/68‑1 (MQ=255) gAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAg < 1:2073527/65‑1 (MQ=255) aCGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTc > 1:3157003/1‑69 (MQ=255) aCGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTc > 1:1006955/1‑69 (MQ=255) aCGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTc > 1:799569/1‑69 (MQ=255) aCGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTc > 1:621499/1‑69 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:866105/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:1113898/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:415687/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:1026492/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:95644/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:1268833/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:1385205/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:3014922/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:1435967/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:2741722/69‑1 (MQ=255) tCGGAATGACGTTGTGGATCAAACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGgag < 1:1569188/69‑1 (MQ=255) aaTGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGat < 1:3387266/69‑1 (MQ=255) aaTGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGat < 1:2179153/69‑1 (MQ=255) aaTGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGat < 1:3203512/69‑1 (MQ=255) aaTGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGat < 1:1679712/69‑1 (MQ=255) aaTGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGat < 1:1429492/69‑1 (MQ=255) aTGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGata < 1:1930666/69‑1 (MQ=255) aTGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGata < 1:1354699/69‑1 (MQ=255) gACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATc < 1:1539753/69‑1 (MQ=255) aCGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATcc > 1:1039698/1‑69 (MQ=255) aCGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATcc > 1:2736332/1‑69 (MQ=255) aCGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATcc > 1:1695828/1‑69 (MQ=255) cAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATCCACCGCGTCGa > 1:3238222/1‑68 (MQ=255) cAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATCCACCGCGTCGAc > 1:1155707/1‑69 (MQ=255) gACCGTGTTCTTCGATGTTCGGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATCCAc < 1:205505/58‑1 (MQ=255) | CGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCAACCGCCAGCGCGTCTGGAGAGATATCCACCGCGTCGAC > minE/1533089‑1533218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |