| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,534,073 | G→C | 33.3% | V72V (GTG→GTC) | yfcN → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,534,073 | 0 | G | C | 33.3% | 84.0 / 64.4 | 84 | V72V (GTG→GTC) | yfcN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (25/31); new base C (21/7); total (46/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCGAT > minE/1534007‑1534130 | acAGGCCGATGCCAGCCACTATTTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa < 1:1514270/68‑1 (MQ=255)acAGGCCGATGCCAGCCACTATTTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa < 1:2584306/68‑1 (MQ=255)acAGGCCGATGCCAGCCACTATTTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa < 1:3106075/68‑1 (MQ=255) cAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGaaa < 1:1333011/69‑1 (MQ=255) cAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGaaa < 1:2199187/69‑1 (MQ=255) aGGCCGATGCCAGCCACTATTTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa < 1:337820/67‑1 (MQ=255) gCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAatat < 1:1551542/69‑1 (MQ=255) gCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAatat < 1:1593718/69‑1 (MQ=255) gCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAatat < 1:2186767/69‑1 (MQ=255) gCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAatat < 1:2190577/69‑1 (MQ=255) cTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAatat < 1:1869473/68‑1 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:2199969/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:2218377/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:2224488/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:3034801/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:3095200/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:2965009/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:2438598/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:1729705/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:2484031/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:2661651/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:1072854/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:383993/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:128879/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:973003/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:86578/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:1348984/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:1365489/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:1412495/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:583138/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:1485954/1‑49 (MQ=255) attTTTCGGACGAATTTCAGCCATTATTGAATACCGAAGGTCCGGTCaa > 1:1149314/1‑49 (MQ=255) attTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCa > 1:1433596/1‑69 (MQ=255) attTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCa > 1:3201637/1‑69 (MQ=255) attTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCa > 1:2737347/1‑69 (MQ=255) attTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCa > 1:162614/1‑69 (MQ=255) attTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCa > 1:1188298/1‑69 (MQ=255) attTCTCCGATGAGTTTCAGCCGGTATTAAATACCGAAGGTCCGGTg > 1:2533740/1‑47 (MQ=255) tcCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCa < 1:3212765/68‑1 (MQ=255) gTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCg < 1:1695137/49‑1 (MQ=255) ttCAGCCATTATTGAATACCGAAGGTCCGGTCAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCg < 1:1362953/68‑1 (MQ=255) ttCAGCCATTATTGAATACCGAAGGTCCGGTCAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCg < 1:2707855/68‑1 (MQ=255) ttCAGCCATTATTGAATACCGAAGGTCCGGTCAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCg < 1:3063179/68‑1 (MQ=255) gCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:2565434/68‑1 (MQ=255) gCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:2696780/68‑1 (MQ=255) gCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:3074387/68‑1 (MQ=255) gCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:1346116/68‑1 (MQ=255) gCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:863651/68‑1 (MQ=255) gCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:1429718/68‑1 (MQ=255) gCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:1619204/68‑1 (MQ=255) gCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:1615601/68‑1 (MQ=255) ccGTTTTTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaagaa < 1:1154672/68‑1 (MQ=255) ccGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaagaa < 1:2504684/68‑1 (MQ=255) ccGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaagaa < 1:1779427/68‑1 (MQ=255) ccGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaagaa < 1:2607978/68‑1 (MQ=255) ccGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaagaa < 1:1510733/68‑1 (MQ=255) ccGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:1113906/67‑1 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:3137782/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:1200591/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:774439/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:709485/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:1438039/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:520871/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:1788819/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:1859499/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:2282599/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:259949/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:281739/1‑69 (MQ=255) attaAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTgcg > 1:2940162/1‑69 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:2431136/54‑1 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:1337647/54‑1 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:2444715/54‑1 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:525383/54‑1 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:2833084/54‑1 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:1954245/54‑1 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCgaaga < 1:2316064/54‑1 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCg > 1:2913065/1‑69 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCg > 1:1538934/1‑69 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCg > 1:2704072/1‑69 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCg > 1:2048704/1‑69 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCg > 1:2761621/1‑69 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCg > 1:2147620/1‑69 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCg > 1:1381999/1‑69 (MQ=255) ccGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCg > 1:2282250/1‑69 (MQ=255) gAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCGat < 1:760823/69‑1 (MQ=255) | GCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGGTGAAATATGTTCGCCCGGATGTCAGCCATTTTGAGGCGAAGAAACTGCGCCGTGGCGAT > minE/1534007‑1534130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |