| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,542,575 | T→G | 46.0% | I613L (ATC→CTC) | yfcX ← | fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,542,575 | 0 | T | G | 46.0% | ‑6.2 / 41.0 | 37 | I613L (ATC→CTC) | yfcX | fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Reads supporting (aligned to +/- strand): ref base T (9/11); new base G (11/6); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01 | |||||||||||
AACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCC > minE/1542510‑1542643 | aaCACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg > 1:1049020/1‑69 (MQ=255)aaCACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg > 1:380510/1‑69 (MQ=255)aaCACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg > 1:406281/1‑69 (MQ=255)aaCACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg > 1:3296161/1‑69 (MQ=255)aaCACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg > 1:2700751/1‑69 (MQ=255)aaCACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg > 1:1905980/1‑69 (MQ=255)aaCACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg > 1:929427/1‑69 (MQ=255)aaCACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCg > 1:1813086/1‑69 (MQ=255) cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGc < 1:393783/68‑1 (MQ=255) cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGc < 1:551019/68‑1 (MQ=255) aCTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCt > 1:2183134/1‑69 (MQ=255) aCTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCt > 1:2780468/1‑69 (MQ=255) aCTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCt > 1:2827088/1‑69 (MQ=255) aCTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCt > 1:150218/1‑69 (MQ=255) aCTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCt > 1:3233005/1‑69 (MQ=255) aCTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCt > 1:1887906/1‑69 (MQ=255) cTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTtgtg > 1:1346963/1‑69 (MQ=255) cTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTtgtg > 1:1530009/1‑69 (MQ=255) cTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTtgtg > 1:2457504/1‑69 (MQ=255) cTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTtgtg > 1:653415/1‑69 (MQ=255) cTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTtgt > 1:1660329/1‑68 (MQ=255) aaCATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCgg > 1:1002690/1‑68 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTTTGTGCCAATCAGCGGGTAAATGGCggg < 1:354106/69‑1 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg < 1:1105553/69‑1 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg < 1:543430/69‑1 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg < 1:2940720/69‑1 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg < 1:2817549/69‑1 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg < 1:272613/69‑1 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg < 1:936101/69‑1 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg < 1:1457804/69‑1 (MQ=255) ccGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCggg < 1:233247/69‑1 (MQ=255) ttCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTTGTGCGCCAATGAGCGGGTAAATGGCGGGATc < 1:80198/69‑1 (MQ=255) ttCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTTGTGCGCCAATGAGCGGGTAAATGGCGGGATc < 1:49859/69‑1 (MQ=255) ttCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTTGTGCGCCAATGAGCGGGTAAATGGCGGGATc < 1:2029600/69‑1 (MQ=255) ttCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTTGTGCGCCAATGAGCGGGTAAATGGCGGGATc < 1:84131/69‑1 (MQ=255) ttCAGCAACCTGCGGTGCGGAGAGTCGCCCCTGCCCTTGTGCGCCAATGAGCGGGTAAATGGCGGGATc < 1:1083789/69‑1 (MQ=255) ttCAGCAACCTGCGGTGCGGAGAGTCGCACCTGCCCTTGTGCGCCAATGAGCGGGTAAATGGCGGGATc < 1:3264353/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:953813/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:3393105/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:3388186/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:2709121/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:25429/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:2493824/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:2062463/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:2019823/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:1847397/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:1711490/69‑1 (MQ=255) ttCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGcc < 1:1660194/69‑1 (MQ=255) | AACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCC > minE/1542510‑1542643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |