| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,542,969 | A→G | 42.3% | G481G (GGT→GGC) | yfcX ← | fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,542,969 | 0 | A | G | 42.3% | 15.0 / 42.9 | 59 | G481G (GGT→GGC) | yfcX | fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Reads supporting (aligned to +/- strand): ref base A (26/8); new base G (19/6); total (45/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
AATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGAATA > minE/1542903‑1543035 | aaTGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTAcc > 1:2603046/1‑69 (MQ=255)aaTGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTAcc > 1:1876937/1‑69 (MQ=255) tAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGt > 1:2542564/1‑69 (MQ=255) tAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGt > 1:935674/1‑69 (MQ=255) tAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGt > 1:669733/1‑69 (MQ=255) tAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGt > 1:1472720/1‑69 (MQ=255) aGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGttt > 1:985332/1‑69 (MQ=255) aGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGttt > 1:64543/1‑69 (MQ=255) aaaaTGCGATTGACGTAAAACCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGcc < 1:1594046/66‑1 (MQ=255) aaaaTGCGATTGACGTAAAACCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGcc < 1:2235984/66‑1 (MQ=255) aaaaTGCGATTGACGTAAAACCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGcc < 1:1807219/66‑1 (MQ=255) aaaaTGCGATTGACGTAAAACCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGcc < 1:419576/66‑1 (MQ=255) aaaaTGCGATTGACGTAAAACCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGcc < 1:850202/66‑1 (MQ=255) aaaaTGCGATTGACGTAAAACCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGcc < 1:127989/66‑1 (MQ=255) gACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGtttt > 1:294478/1‑64 (MQ=255) gACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACtgt > 1:3081777/1‑69 (MQ=255) gACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACtgt > 1:100630/1‑69 (MQ=255) gACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACtgt > 1:2436774/1‑69 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCTGTTTTACTGTGGTa > 1:2302/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGt > 1:1591839/1‑67 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:3179390/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:2683530/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:3124138/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:3401350/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:2765773/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:2667818/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:987926/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:1242514/1‑68 (MQ=255) aaaacCCGGCTTTGTCACGCACGACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTa > 1:1535816/1‑68 (MQ=255) aaaaCCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGc > 1:2199981/1‑69 (MQ=255) aaaaCCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGc > 1:762101/1‑69 (MQ=255) aaaaCCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGc > 1:1851330/1‑69 (MQ=255) aaaaCCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGc > 1:1709498/1‑69 (MQ=255) aaaaCCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGc > 1:153256/1‑69 (MQ=255) tttGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTg < 1:371909/69‑1 (MQ=255) cacgcacgACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGcc < 1:7240/68‑1 (MQ=255) cacgcacgACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGcc < 1:1473321/68‑1 (MQ=255) cacgcacgACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGcc < 1:519301/68‑1 (MQ=255) gcacgACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTgcg > 1:2053032/1‑63 (MQ=255) gcacgACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATg > 1:2325530/1‑69 (MQ=255) gcacgACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATg > 1:957165/1‑69 (MQ=255) gcacgACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATg > 1:454040/1‑69 (MQ=255) gcacgACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATg > 1:3258680/1‑69 (MQ=255) gcacgACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATg > 1:2857139/1‑69 (MQ=255) gcacgACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATg > 1:2989547/1‑69 (MQ=255) gcacgACAATGGGCGTTTTGCCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGAGCCGATg > 1:1388735/1‑69 (MQ=255) gACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTccc < 1:1564015/69‑1 (MQ=255) gACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTccc < 1:3062167/69‑1 (MQ=255) gACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTccc < 1:303512/69‑1 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAgg < 1:2937067/65‑1 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaata > 1:3349762/1‑69 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaata > 1:3161421/1‑69 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaata > 1:76299/1‑69 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaata > 1:828603/1‑69 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaata > 1:1369425/1‑69 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaata > 1:1318986/1‑69 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaata > 1:1067842/1‑69 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaat > 1:2277139/1‑68 (MQ=255) ttACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGaat > 1:2568356/1‑68 (MQ=255) | AATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATGGTTTGCGCCGATGTCCCCGCATGAGGAATA > minE/1542903‑1543035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |