| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,545,006 | A→G | 100% | V239A (GTC→GCC) | yfcY ← | beta‑ketoacyl‑CoA thiolase, anaerobic, subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,545,006 | 0 | A | G | 77.2% | 87.5 / 16.5 | 65 | V239A (GTC→GCC) | yfcY | beta‑ketoacyl‑CoA thiolase, anaerobic, subunit |
| Reads supporting (aligned to +/- strand): ref base A (14/1); new base G (21/29); total (35/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAC > minE/1544968‑1545071 | tagCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:1586503/66‑1 (MQ=255)cagCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:3327845/66‑1 (MQ=255)cagCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:825358/66‑1 (MQ=255)cagCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:1239470/66‑1 (MQ=255)cagCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:1824042/66‑1 (MQ=255) agCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:419728/66‑1 (MQ=255) agCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:3230011/66‑1 (MQ=255) agCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:1532572/66‑1 (MQ=255) agCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:456914/66‑1 (MQ=255) agCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:787273/66‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2628046/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:1089250/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2638511/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2513837/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2651812/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2660997/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2019242/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2225949/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2945077/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:2147860/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:3289810/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:3392774/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:1541741/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:1431450/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:1329351/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:579281/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:592911/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGGCAAGCGGTTGTTTATAag < 1:1193045/41‑1 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAATGCAGTCATCACCtcttc > 1:1609968/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtcttc > 1:2728335/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtcttc > 1:255651/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtcttc > 1:1123486/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtcttc > 1:1778167/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtcttc > 1:1219751/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtcttc > 1:1325864/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtcttc > 1:1243246/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtcttc > 1:1427316/1‑69 (MQ=255) aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGAAAAAGGCAGTCATCACct > 1:1283110/1‑65 (MQ=255) aaTATTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATAAAg < 1:2471115/46‑1 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:3401424/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:3045909/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:3068037/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:586443/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:1718502/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:582892/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:1583551/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:2816421/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:1323342/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:578396/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:2980623/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:1885758/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:1982646/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:2005045/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:2555241/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:2473736/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:945948/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaaa > 1:2101940/1‑43 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATaa > 1:654927/1‑42 (MQ=255) tatTGTTGTCTTCGGCAAGCGGTTGTTTATAAGGAGGGATa > 1:575781/1‑41 (MQ=255) tatTGTTGTCTTCGGAAAGCGGTTGTTTATAAGGAGGGATaaa > 1:1877570/1‑43 (MQ=255) gtCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCg < 1:1835101/67‑1 (MQ=255) ttCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTg > 1:2100979/1‑68 (MQ=255) ttCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTg > 1:678349/1‑68 (MQ=255) ttCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTg > 1:3277292/1‑68 (MQ=255) ttCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTg > 1:223259/1‑68 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:561988/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:1664592/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:1733085/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:2776013/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:1943639/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:201249/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:854338/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:870954/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:2020807/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:960748/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:967227/66‑1 (MQ=255) aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAc < 1:971239/66‑1 (MQ=255) | CGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAC > minE/1544968‑1545071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |