Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,551,248 G→T 48.6% V232V (GTG→GTT yfdC → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,551,2480GT48.6% 11.2 / 78.4 72V232V (GTG→GTTyfdCpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (30/7);  new base T (19/16);  total (49/23)
Fisher's exact test for biased strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

TATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCT  >  minE/1551200‑1551312
                                                |                                                                
tataTTGATGAGCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:1894895/1‑51 (MQ=38)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:1087062/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:2559541/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:2840042/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:2852831/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:287287/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:3007646/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:2070151/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:3097568/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:197238/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:187541/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:3284843/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:1762538/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:1644222/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:1557538/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:770646/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGt                                                                >  1:618341/1‑51 (MQ=39)
tataTTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCAAATCGTTGt                                                                >  1:1837768/1‑51 (MQ=255)
tataTTGATGACCTGGCTTATTGCCCTGGGTGAAACCACCCACATCGTTGt                                                                >  1:3125813/1‑51 (MQ=255)
      gatgaCCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATc                                         >  1:1199766/1‑68 (MQ=255)
      gatgaCCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCt                                        >  1:1559411/1‑69 (MQ=255)
      gatgaCCTGGCTTATTGCCCTGGGTGACAACACCCATATCGTGGTAGGTTCTGTTGAAATCCTCTATCt                                        >  1:1420383/1‑69 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:3272536/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:3068350/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:667460/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:423297/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:3356695/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:790027/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:869956/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:2751828/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:2444940/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:2431014/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:1768102/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:1985536/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:2007928/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:1198337/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:2088766/69‑1 (MQ=255)
             tGGCTTATTGCCCTGGGTGACACCACCCACATCGTTGTCGGTTCTGTTGAAATCCTCTATCTGGTGttt                                 <  1:2330743/69‑1 (MQ=255)
                                acacCACCCATATCGTGGTCGGTTCTGTTGAAATCctct                                            >  1:979711/1‑39 (MQ=255)
                                acacCACCCATATCGTGGTCGGTTCTGTTGAAATCctct                                            >  1:2182528/1‑39 (MQ=255)
                                acacCACCCATATCGTGGTCGGTTCTGTTGAAATCctct                                            >  1:1030465/1‑39 (MQ=255)
                                acacCACCCATATCGTGGTCGGTTCTGTTGAAATCctct                                            >  1:2625398/1‑39 (MQ=255)
                                acacCACCCATATCGTGGTCGGTTCTGTTGAAATCctct                                            >  1:250337/1‑39 (MQ=255)
                                acacCACCCATATCGTGGTCGGTTCTGTTGAAATCctct                                            >  1:629903/1‑39 (MQ=255)
                                acacCACCCATATCGTGGTCGGTTCTGTTGAAATCctc                                             >  1:2816390/1‑38 (MQ=255)
                                     accCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGAt         <  1:2945211/69‑1 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                              >  1:473340/1‑44 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                              >  1:374321/1‑44 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                              >  1:1399915/1‑44 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                              >  1:1954337/1‑44 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                              >  1:2411801/1‑44 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                              >  1:1137546/1‑44 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAAc                              >  1:861980/1‑44 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCa     <  1:1305873/69‑1 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCa     <  1:58421/69‑1 (MQ=255)
                                         atatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCa     <  1:289458/69‑1 (MQ=255)
                                          tatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCAt    <  1:2424055/69‑1 (MQ=255)
                                          tatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCAt    <  1:2913032/69‑1 (MQ=255)
                                          tatCGTGGTCGGTTCTGTTGAAATCCTCTATCTGTGTTTAACGGTACGCTGCACTGGAGCGATTTCAt    <  1:1113104/68‑1 (MQ=255)
                                            tCGTGGTCTGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:2250203/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATc   >  1:2008382/1‑68 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:1524477/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:402817/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:3415868/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:613521/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:1535032/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:3228953/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:3176639/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:741983/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:3057890/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:851844/1‑69 (MQ=255)
                                            tCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCt  >  1:997604/1‑69 (MQ=255)
                                                |                                                                
TATATTGATGACCTGGCTTATTGCCCTGGGTGACACCACCCATATCGTGGTCGGTTCTGTTGAAATCCTCTATCTGGTGTTTAACGGTACGCTGCACTGGAGCGATTTCATCT  >  minE/1551200‑1551312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: