| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,552,932 | A→C | 31.9% | T106T (ACT→ACG) | dsdC ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,552,932 | 0 | A | C | 31.9% | 38.5 / 32.3 | 47 | T106T (ACT→ACG) | dsdC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (20/12); new base C (6/9); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCG > minE/1552872‑1552996 | tgtAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGTGAATACAGAGTTAACGt < 1:2638736/69‑1 (MQ=255)tgtAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGt < 1:614567/69‑1 (MQ=255)tgtAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGt < 1:2701503/69‑1 (MQ=255)tgtAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGt < 1:1507395/69‑1 (MQ=255)tgtAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGt < 1:1462958/69‑1 (MQ=255)tgtAAAGGCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGt < 1:1005891/69‑1 (MQ=255) gtAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGt < 1:2568872/68‑1 (MQ=255) tAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTc > 1:1691634/1‑69 (MQ=255) tCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGAt < 1:1630420/69‑1 (MQ=255) tCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGAt < 1:1856777/69‑1 (MQ=255) tCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGAt < 1:2110526/69‑1 (MQ=255) tCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGAt < 1:2030389/69‑1 (MQ=255) cGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:2890158/69‑1 (MQ=255) cGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:3196431/69‑1 (MQ=255) cGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:513097/69‑1 (MQ=255) cGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:86476/69‑1 (MQ=255) cGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:2576386/69‑1 (MQ=255) cGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:1393424/69‑1 (MQ=255) cGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:1556558/69‑1 (MQ=255) gggCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:2275083/68‑1 (MQ=255) ggCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGAttt > 1:2164752/1‑69 (MQ=255) ggCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGAttt > 1:1417471/1‑69 (MQ=255) gCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGCGTTAACGTTCCCGATAACTCCTGAt < 1:1893151/66‑1 (MQ=255) gggCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAAttt > 1:1011544/1‑69 (MQ=255) gggCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAAttt > 1:3198595/1‑69 (MQ=255) gggCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAAttt > 1:3109428/1‑69 (MQ=255) gggCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAAttt > 1:2569901/1‑69 (MQ=255) gggCGATAGAGGGCCGGGAATACAGAGTTAACGTGCCCGATAACTCCTGATTTTTGATATCAAGAAttt > 1:726565/1‑69 (MQ=255) cGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTa < 1:2889070/69‑1 (MQ=255) aTACAGCGTTAACGTTCCCGATAACTCCTGATTTTTGATATCCAGAATTTCCTGATTCAGGGTATCCa > 1:2232318/1‑68 (MQ=255) aTACAGCGTTAACGTTCCCGATAACTCCTGATTTTTGATATCCAGAATTTCCTGATTCAGGGTATCCa > 1:2020303/1‑68 (MQ=255) aTACAGCGTTAACGTTCCCGATAACTCCTGATTTTTGATATCCAGAATTTCCTGATTCAGGGTATCCa > 1:672746/1‑68 (MQ=255) aTACAGCGTTAACGTTCCCGATAACTCCTGATTTTTGATATCCAGAATTTCCTGATTCAGGGTATCCa > 1:545566/1‑68 (MQ=255) aTACAGCGTTAACGTTCCCGATAACTCCTGATTTTTGATATCCAGAATTTCCTGATTCAGGGTATCCa > 1:154222/1‑68 (MQ=255) aTACAGCGTTAACGTTCCCGATAACTCCTGAATTTTGATATCCAGAATTTCCTgat > 1:2768461/1‑54 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAAt > 1:2668482/1‑45 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:2905196/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:1722480/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:2632796/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:2091261/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:3363581/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:3411809/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:1310683/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:593937/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGcg > 1:1547579/1‑68 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGc > 1:3192225/1‑67 (MQ=255) cAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGc > 1:1236816/1‑67 (MQ=255) | TGTAAAGTCACCTAATGCGGGCACCAACCAGCATTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATAACTCCTGATTTTTGATATCAAGAATTTCCTGGTTCAGGGTATCCAGCG > minE/1552872‑1552996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |