| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 123,017 | G→A | 68.0% | G399S (GGC→AGC) | cueO → | multicopper oxidase (laccase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 123,017 | 0 | G | A | 68.0% | 87.0 / 55.4 | 106 | G399S (GGC→AGC) | cueO | multicopper oxidase (laccase) |
| Reads supporting (aligned to +/- strand): ref base G (22/12); new base A (38/34); total (60/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GGATCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGAT > minE/122959‑123084 | ggatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGtt < 1:3391515/69‑1 (MQ=255)ggatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGtt < 1:1618223/69‑1 (MQ=255)ggatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGtt < 1:3058714/69‑1 (MQ=255)ggatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGAGGGAAGtt < 1:3075918/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACTGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:2393866/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTc > 1:3273021/1‑69 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGtt < 1:2925880/68‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGtt < 1:2755259/68‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGtt < 1:2443760/68‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGtt < 1:2176188/68‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:484395/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:3126417/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:2786958/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:249521/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:716559/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:2029661/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:1957384/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:1667478/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:2220926/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:1009285/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:806234/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:1467363/69‑1 (MQ=255) gatCACAGCCAGATGATGGGCCATATGGGGCACGACAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:1545529/69‑1 (MQ=255) atCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:485229/68‑1 (MQ=255) atCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:2854441/68‑1 (MQ=255) atCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:1755146/68‑1 (MQ=255) tgatgGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTccacca > 1:1815653/1‑67 (MQ=255) tgatgGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCAt > 1:1856592/1‑68 (MQ=255) tgatgGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCAt > 1:2093885/1‑68 (MQ=255) tgatgGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCAt > 1:1667383/1‑68 (MQ=255) cATATGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:107865/48‑1 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCg > 1:1427091/1‑48 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTccacca > 1:511775/1‑58 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTc > 1:309261/1‑53 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTc > 1:2640384/1‑53 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:2573985/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:3249406/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:912949/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:128899/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:2858804/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:2909124/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:3404958/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:1179103/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:2961381/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:1626854/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:1071865/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:951302/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:1472143/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:1573233/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:736397/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:827312/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:2411006/1‑59 (MQ=255) atatGGGGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTCGATTTCCACCAt > 1:133957/1‑59 (MQ=255) tGGTGCACGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:497334/44‑1 (MQ=38) tGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATc > 1:1989777/1‑68 (MQ=255) tGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCa > 1:784100/1‑69 (MQ=255) gggCACGGCAATATGAATCATATGAACAACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAAc > 1:2102821/1‑69 (MQ=255) ggCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACg > 1:3007289/1‑69 (MQ=255) ggCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACg > 1:31671/1‑69 (MQ=255) ggCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACg > 1:1111007/1‑69 (MQ=255) cGGCAATATGAATCATATGAACCACAGCGGGAAGTTc < 1:3244592/37‑1 (MQ=255) gCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATc < 1:2455475/59‑1 (MQ=255) atGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACg > 1:529014/1‑58 (MQ=255) atGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACg > 1:487547/1‑58 (MQ=255) atGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACg > 1:1702399/1‑58 (MQ=255) atGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGttt < 1:640712/69‑1 (MQ=255) atGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGttt < 1:1682858/69‑1 (MQ=255) atGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGttt < 1:713942/69‑1 (MQ=255) atGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGttt < 1:25613/69‑1 (MQ=255) tGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGttt < 1:971746/68‑1 (MQ=255) tGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGttt < 1:2946037/68‑1 (MQ=255) tGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGttt > 1:1335535/1‑68 (MQ=255) tGAATCATATGAACCACGGCGGGAAGTTAGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGttt > 1:1846811/1‑68 (MQ=255) aaTCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGa < 1:73789/68‑1 (MQ=255) aaTCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGa < 1:404526/68‑1 (MQ=255) aaTCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGa < 1:1040621/68‑1 (MQ=255) aaTCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGa < 1:1412440/68‑1 (MQ=255) aaTCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGa < 1:2905632/68‑1 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGatat > 1:1122908/1‑68 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGatat > 1:1310097/1‑68 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:245123/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:1155207/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:1569198/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:192956/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:2358959/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:3316454/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:2473314/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:2792244/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:2921691/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:3313904/1‑69 (MQ=255) cATATGAACCACAGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:404494/1‑69 (MQ=255) atatGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATg > 1:3192701/1‑68 (MQ=255) aCGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGa > 1:2965176/1‑69 (MQ=255) cggcggGAAGTTCGATTTCCACCATGCCAACAAAATc < 1:672034/37‑1 (MQ=255) cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGAt < 1:2743310/69‑1 (MQ=255) cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGAt < 1:1939467/69‑1 (MQ=255) cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGAt < 1:739006/69‑1 (MQ=255) cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGAt < 1:1692356/69‑1 (MQ=255) cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGAt < 1:801129/69‑1 (MQ=255) aggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGAt > 1:598378/2‑69 (MQ=255) | GGATCACAGCCAGATGATGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGAT > minE/122959‑123084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |