| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,553,364 | A→C | 49.4% | intergenic (‑115/‑103) | dsdC ← / → dsdX | DNA‑binding transcriptional dual regulator/predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,553,364 | 0 | A | C | 49.4% | 19.4 / 200.8 | 154 | intergenic (‑115/‑103) | dsdC/dsdX | DNA‑binding transcriptional dual regulator/predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (69/9); new base C (16/60); total (85/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.09e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01 | |||||||||||
Only 100 of 154 total aligned reads displayed.
AGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCC > minE/1553305‑1553431 | agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:2857744/1‑69 (MQ=255)agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:3301967/1‑69 (MQ=255)agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:2514393/1‑69 (MQ=255)agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:1639761/1‑69 (MQ=255)agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:2053566/1‑69 (MQ=255)agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:1602684/1‑69 (MQ=255)agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:2592839/1‑69 (MQ=255)agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:1222176/1‑69 (MQ=255)agatgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCaa > 1:1410221/1‑69 (MQ=255) tgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAAttt > 1:2074727/1‑69 (MQ=255) tgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAAttt > 1:3420085/1‑69 (MQ=255) tgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAAttt > 1:2304907/1‑69 (MQ=255) tgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAAttt > 1:126620/1‑69 (MQ=255) tgagCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAAttt > 1:3154496/1‑69 (MQ=255) tAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAAttt < 1:2343734/64‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:2100861/69‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:636096/69‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:3260198/69‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:2763993/69‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:47763/69‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:2803763/69‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:951485/69‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:1069821/69‑1 (MQ=255) aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:3262941/69‑1 (MQ=255) tctcAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:1492768/56‑1 (MQ=255) tctcAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:2391064/56‑1 (MQ=255) tctcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCaaa > 1:3062288/1‑69 (MQ=255) tctcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCaaa > 1:2322632/1‑69 (MQ=255) tcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAAtt > 1:2556496/1‑47 (MQ=255) tcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCaaa > 1:2121386/1‑67 (MQ=255) tcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAAt > 1:2389676/1‑68 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2545400/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:3034608/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2504551/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2444326/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1929046/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1119119/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:809922/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1151124/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1292661/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:681269/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:552025/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:404660/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:3301101/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1901096/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2017053/66‑1 (MQ=255) cACCTTCATTTTTAGACGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg < 1:799680/69‑1 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:600120/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:741435/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:75954/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:96495/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:2433065/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:1035314/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:1745220/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:1662736/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:208868/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:1120073/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:2264939/1‑69 (MQ=255) tttttAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCa < 1:2374835/68‑1 (MQ=255) tttttAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCa < 1:2082301/68‑1 (MQ=255) tttAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAg < 1:1405881/67‑1 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAAt > 1:3010484/1‑44 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:1072319/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:166557/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:3156003/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:1457208/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:1433383/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:2363570/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:1213934/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAAtttt > 1:1008888/1‑45 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg > 1:2152268/1‑49 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg > 1:25838/1‑49 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg > 1:172752/1‑49 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:476889/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:370475/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:3385586/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:3349662/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:1792528/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:2803110/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:3111759/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:2007400/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:314651/1‑69 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:1807624/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:2722322/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:920659/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:2173326/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:3224158/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:401386/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:1977095/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:3273330/69‑1 (MQ=255) cACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGtttttt > 1:1162339/1‑69 (MQ=255) cACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGtttttt > 1:3226519/1‑69 (MQ=255) ccATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGt > 1:2167439/1‑68 (MQ=255) ccATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGt > 1:806068/1‑68 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:148354/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:570513/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:3130108/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:1261157/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:924637/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:2408842/1‑69 (MQ=255) | AGATGAGCTAAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCC > minE/1553305‑1553431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |