| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,553,378 | C→G | 52.7% | intergenic (‑129/‑89) | dsdC ← / → dsdX | DNA‑binding transcriptional dual regulator/predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,553,378 | 0 | C | G | 52.7% | 16.4 / 180.2 | 146 | intergenic (‑129/‑89) | dsdC/dsdX | DNA‑binding transcriptional dual regulator/predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (59/10); new base G (16/61); total (75/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.34e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
Only 100 of 146 total aligned reads displayed.
AAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATA > minE/1553314‑1553435 | aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:956443/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:2100861/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:2801895/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:2803763/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:3250446/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:3260198/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:398466/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:47763/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:636096/69‑1 (MQ=255)aaaGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:1227426/69‑1 (MQ=255) tctcAATTCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCa < 1:1492768/56‑1 (MQ=255) tctcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCaaa > 1:2394433/1‑69 (MQ=255) tcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCaaa > 1:2121386/1‑67 (MQ=255) tcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCaaa > 1:2521740/1‑67 (MQ=255) tcAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAAt > 1:2389676/1‑68 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:681269/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2444326/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2017053/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2504551/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1929046/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1901096/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2545400/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:2693751/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:920906/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:809922/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:3034608/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:3146542/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1112508/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:404660/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1415862/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1369182/66‑1 (MQ=255) ttCACCTTCATTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAAttt < 1:1292661/66‑1 (MQ=255) cACCTTCATTTTTAGACGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg < 1:799680/69‑1 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:3122646/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:75954/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:741435/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:2433065/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:96495/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:600120/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:2264939/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:1035314/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:1120073/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:1134599/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:1211483/1‑69 (MQ=255) aTTTTTAGATGTAAATCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCa > 1:208868/1‑69 (MQ=255) tttttAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCa < 1:2082301/68‑1 (MQ=255) ttttAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCa < 1:1519486/67‑1 (MQ=255) tttAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAg < 1:1405881/67‑1 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAAt > 1:3010484/1‑44 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGc > 1:2375993/1‑64 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:1072319/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:2363570/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:612234/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:1457208/1‑69 (MQ=255) tAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttgt > 1:166557/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAAtttt > 1:1008888/1‑45 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg > 1:2152268/1‑49 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg > 1:2817367/1‑49 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg > 1:172752/1‑49 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCg > 1:25838/1‑49 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCgttg > 1:1469433/1‑66 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:3349662/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:3385586/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:3285128/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:370475/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:422838/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:314651/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:3111759/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:3051062/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:1792528/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:2007400/1‑69 (MQ=255) aaTCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttt > 1:1770764/1‑69 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:1977095/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:2357050/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:1807624/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:2722322/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:3273330/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:1039773/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:3224158/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:3176663/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:3344485/69‑1 (MQ=255) tCACTCCCTTGATGCAATTTAGCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:2173326/69‑1 (MQ=255) cACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGtttttt > 1:1162339/1‑69 (MQ=255) ccATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGt > 1:2167439/1‑68 (MQ=255) ccATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGt > 1:806068/1‑68 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:1261157/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:570513/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:2408842/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:479131/1‑69 (MQ=255) cATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTcc > 1:924637/1‑69 (MQ=255) tGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAat < 1:1720436/69‑1 (MQ=255) gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAat > 1:890510/1‑68 (MQ=255) gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAat > 1:2773012/1‑68 (MQ=255) gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAat > 1:1298671/1‑68 (MQ=255) gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAat > 1:591120/1‑68 (MQ=255) gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAat > 1:2614655/1‑68 (MQ=255) gATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAat > 1:167471/1‑68 (MQ=255) aTGCAATTTAGCTCTTGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGttttt < 1:1764027/58‑1 (MQ=255) tACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAata > 1:651137/1‑60 (MQ=255) tACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAata > 1:2243405/1‑60 (MQ=255) | AAAGTGAACCATATCTCAATTCACCTTCATTTTTAGATGTAAATCACTCCATTGATGCAATTTACCTCATGTGAAAGGCAAATTTTATCGTTTGTCAGCCTGCGTTGTTTTTTTGTCCAATA > minE/1553314‑1553435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |