BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,554,687	A→G	34.8%	L407L (CTA→CTG)	dsdX →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,554,687	0	A	G	34.8%	75.5 / 64.1	109	L407L (CTA→CTG)	dsdX	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (14/57); new base G (18/20); total (32/77)
Fisher's exact test for biased strand distribution p-value = 3.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

ATTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATACTATAC  >  minE/1554628‑1554737

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:1016768/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:1746683/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:1801004/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:206245/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:2213186/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:2256919/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:2409772/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:2631686/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:2853553/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:467597/69‑1 (MQ=255)

aTTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:364095/69‑1 (MQ=255)

      aTCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTgc                                     <  1:2802810/69‑1 (MQ=255)

             cAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGc                                             <  1:1095238/54‑1 (MQ=255)

             cAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGc                                             <  1:2163767/54‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCTCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:2287596/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAATCaa                                           <  1:2449068/51‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         >  1:600593/1‑69 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:290237/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:2057330/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:3354189/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:3237320/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:2290825/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:2405390/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:2556132/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:3042647/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:2752924/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:1530467/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         >  1:843967/1‑69 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:1128877/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:1450808/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:1071691/69‑1 (MQ=255)

                  gCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCCCTACGCTc                         <  1:1718554/69‑1 (MQ=255)

                   cAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:1637104/68‑1 (MQ=255)

                        ggCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTgc                                     >  1:1614906/1‑51 (MQ=255)

                        ggCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTgc                                     >  1:1911052/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:3403662/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:1079733/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:22473/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:429555/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:2274896/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:1477988/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:1052547/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:13576/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:2436296/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:754875/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:218680/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:2552921/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:2608977/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:3061859/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:747781/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:1824050/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:680091/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTg                                                 <  1:1722091/39‑1 (MQ=37)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAat                                          >  1:2866675/1‑46 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:2896123/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:3158819/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:1103758/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:3370445/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:3392480/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:445285/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:2126157/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:530270/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:2047295/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:2033479/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:2029497/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:1987261/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:1911218/1‑51 (MQ=255)

                        ggCTGCACGATCGTTACGGACTCACTTTTCTGGCTGGTGAAGCAATATTgc                                     >  1:1649345/1‑51 (MQ=255)

                               cTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTa            <  1:1137367/69‑1 (MQ=255)

                                tATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTa            <  1:2490814/68‑1 (MQ=255)

                                 aTCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:2475221/36‑1 (MQ=255)

                                 aTCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCaa                                           <  1:1293324/36‑1 (MQ=255)

                                  tCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAAt         >  1:3274087/1‑69 (MQ=255)

                                  tCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAAt         >  1:3352781/1‑69 (MQ=255)

                                  tCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAAt         >  1:1327541/1‑69 (MQ=255)

                                  tCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAAt         >  1:2280981/1‑69 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTaaa          <  1:1118792/66‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTaaa          <  1:1817485/66‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTaaa          <  1:3050678/66‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTaaa          <  1:823617/66‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTaaa          <  1:2446928/66‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTaaa          <  1:1346413/66‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTaaa          <  1:2384946/66‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:2961740/69‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:78398/69‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:1542047/69‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:1521750/69‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:1493621/69‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:3074268/69‑1 (MQ=255)

                                    gTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGAGCTACGCTCAATGAAACATTTAAATAc       <  1:1099012/69‑1 (MQ=255)

                                     ttACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:1648238/68‑1 (MQ=255)

                                       aCGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTc                         <  1:883154/48‑1 (MQ=255)

                                       aCGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTa            >  1:2752607/1‑61 (MQ=255)

                                       aCGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTa            >  1:2446453/1‑61 (MQ=255)

                                       aCGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTa            >  1:1288112/1‑61 (MQ=255)

                                       aCGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTa            >  1:1072980/1‑61 (MQ=255)

                                       aCGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTa            >  1:1048618/1‑61 (MQ=255)

                                          gACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATACTATAc  <  1:784809/68‑1 (MQ=255)

                                            cTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:577162/61‑1 (MQ=255)

                                            cTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATAc       <  1:1583915/61‑1 (MQ=255)

ATTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTTACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTTAAATACTATAC  >  minE/1554628‑1554737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`