Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,555,145	T→C	79.5%	D108D (GAT→GAC)	dsdA →	D‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,555,145	0	T	C	79.5%	38.7 / 3.6	29	D108D (GAT→GAC)	dsdA	D‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base C (16/7);  total (20/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

CAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGCACACG  >  minE/1555083‑1555209                                                               |                                                                 cAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATCGCCa                                                             <  1:1740049/68‑1 (MQ=255) cAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCa                                                             <  1:3277076/68‑1 (MQ=255)                  aGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCt                                            >  1:908889/1‑68 (MQ=255)                  aGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTc                                           >  1:962514/1‑69 (MQ=255)                                            aaCTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTa                   >  1:1629463/1‑67 (MQ=255)                                            aaCTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTa                   >  1:3296450/1‑67 (MQ=255)                                                        aaaaaTACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGt             >  1:757434/1‑61 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     >  1:1146509/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     >  1:846293/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     >  1:3312805/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     >  1:2680598/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     >  1:1131745/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     >  1:1526967/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     >  1:1856553/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     >  1:1700188/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGcc      >  1:2705092/1‑67 (MQ=255)                                                        aaaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGcc      >  1:2861561/1‑67 (MQ=255)                                                         aaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     <  1:999877/68‑3 (MQ=255)                                                         aaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     <  1:1277331/68‑3 (MQ=255)                                                         aaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     <  1:1606249/68‑3 (MQ=255)                                                         aaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     <  1:346316/68‑3 (MQ=255)                                                         aaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     <  1:3353819/68‑3 (MQ=255)                                                         aaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     <  1:3098251/68‑3 (MQ=255)                                                         aaaaGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGccc     <  1:2445274/68‑3 (MQ=255)                                                            aGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCAcgt                              >  1:2226192/1‑39 (MQ=37)                                                            aGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTg         >  1:2240034/1‑61 (MQ=255)                                                            aGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGcccatg  >  1:1901940/1‑66 (MQ=255)                                                            aGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGcccatg  >  1:3225326/1‑66 (MQ=255)                                                            aGACAGCCATTTGCCGATTTCCGGCTCCATAAAAGCACGTGGCGGGATTTATGAAGTCCTGGcccatg  >  1:1939818/1‑66 (MQ=255)                                                               |                                                                 CAAAAACGGCTGGAAAAAGAATATCAGCAACCGATCAGCGGGCAACTGTTACTGAAAAAAGATAGCCATTTGCCCATTTCCGGCTCCATAAAAGCACGCGGCGGGATTTATGAAGTCCTGGCACACG  >  minE/1555083‑1555209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑