| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,556,173 | A→C | 28.4% | intergenic (+23/+1240) | dsdA → / ← emrY | D‑serine ammonia‑lyase/predicted multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,556,173 | 0 | A | C | 28.4% | 97.5 / 55.3 | 88 | intergenic (+23/+1240) | dsdA/emrY | D‑serine ammonia‑lyase/predicted multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base A (21/42); new base C (3/22); total (24/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GGTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCCGCTATA > minE/1556105‑1556240 | ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:3213687/69‑1 (MQ=255)ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:506272/69‑1 (MQ=255)ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:1504828/69‑1 (MQ=255)ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:2810725/69‑1 (MQ=255)ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:2782654/69‑1 (MQ=255)ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:2450209/69‑1 (MQ=255)ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:2012984/69‑1 (MQ=255)ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:723829/69‑1 (MQ=255)ggTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:2508622/69‑1 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:629783/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:809230/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2434488/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2400361/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:1892407/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:1906781/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:328814/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:640040/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2040806/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2165821/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2146901/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2434854/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:1440481/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2528430/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2680375/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2682113/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:1321423/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:1306374/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2705316/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCaa > 1:2863259/1‑69 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa < 1:1494836/68‑1 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCa > 1:2339745/1‑68 (MQ=255) gTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGATTCAACGCAGCATCGCaa > 1:2337644/1‑69 (MQ=255) ggAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt < 1:2188308/68‑1 (MQ=255) ggAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt < 1:298180/68‑1 (MQ=255) ggAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt < 1:422676/68‑1 (MQ=255) ggAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt < 1:1091856/68‑1 (MQ=255) ggAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt < 1:1357326/68‑1 (MQ=255) gagaTGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTgg > 1:3334624/1‑69 (MQ=255) gagaTGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTgg > 1:1036683/1‑69 (MQ=255) gagaTGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTgg > 1:1812802/1‑69 (MQ=255) cAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAt < 1:3393155/69‑1 (MQ=255) cAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAt < 1:2988535/69‑1 (MQ=255) cAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAt < 1:90920/69‑1 (MQ=255) cAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAt < 1:2877640/69‑1 (MQ=255) cAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAt < 1:2700537/69‑1 (MQ=255) cAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAt < 1:840827/69‑1 (MQ=255) cAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAt < 1:823529/69‑1 (MQ=255) cAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGAt < 1:1571124/69‑1 (MQ=255) tgtcAAAAGGCCGTTAATAACGTTTCAACGCGGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGcc < 1:2363432/65‑1 (MQ=255) tagCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt > 1:2090153/3‑45 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt > 1:2040563/1‑45 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt > 1:370663/1‑45 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt > 1:89740/1‑45 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt > 1:3173559/1‑45 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt > 1:2135623/1‑45 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt > 1:2617231/1‑45 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCt > 1:1276188/1‑45 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGcc < 1:1610326/68‑1 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGcc < 1:540428/68‑1 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGcc < 1:64355/68‑1 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGcc < 1:2086841/68‑1 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGcc < 1:3420976/68‑1 (MQ=255) tGGCAAAAGGCCGTTAATAACGTTTCAACGAAGCATCGCAATCCt > 1:2184572/1‑45 (MQ=255) aaaaGGCGGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:2505849/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:3295597/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:1083237/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:3039960/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:393956/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:1678201/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:1184530/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:2390416/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:2384257/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:787556/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:2011753/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:2723758/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:2799644/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:1188342/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:2022304/67‑1 (MQ=255) aaaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGGGAGCGATGCTGCCGAt < 1:1401376/67‑1 (MQ=255) aaaGGCCGTTAATAACGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGAt < 1:277843/66‑1 (MQ=255) ccGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGa < 1:3308971/69‑1 (MQ=255) ccGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGa < 1:543290/69‑1 (MQ=255) taaCGTTTCAACGCAGCATCGCCATCCTTTCCCTGGGTGAGCGATGCTGCCGATGACGCAGACTTAAGa > 1:1424687/1‑69 (MQ=255) tCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCgg > 1:2174566/1‑69 (MQ=255) tCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGAGGGCGCAGACTTAAGATCCCCgg > 1:2819658/1‑69 (MQ=255) cGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCtt < 1:103984/69‑1 (MQ=255) cGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCtt < 1:2203851/69‑1 (MQ=255) cGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCtt < 1:1964721/69‑1 (MQ=255) cGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCtt < 1:1742327/69‑1 (MQ=255) cGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCtt < 1:1604287/69‑1 (MQ=255) cGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCtt < 1:1437227/69‑1 (MQ=255) cGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCtt < 1:1190813/69‑1 (MQ=255) aTCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATc < 1:2174176/54‑1 (MQ=255) aTCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCCGc < 1:3191610/69‑1 (MQ=255) aTCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCCGc < 1:3036239/69‑1 (MQ=255) aTCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCCGc < 1:2334974/69‑1 (MQ=255) aTCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCCGc < 1:1492439/69‑1 (MQ=255) tCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCCGc < 1:1122236/68‑1 (MQ=255) cAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCtt < 1:928511/59‑1 (MQ=255) aaTCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCCGCtata < 1:2437751/68‑1 (MQ=255) | GGTGCCGGAAGAAGAGATGAATCAATATCTGGCAAAAGGCCGTTAATAACGTTTCAACGCAGCATCGCAATCCTTTCCCTGGGTGAGCGATGCTGCCGATGGCGCAGACTTAAGATCCCCGGTCTTACCCGCTATA > minE/1556105‑1556240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |